Lang_2010_BMB.Rep_43_573

Reference

Title : Molecular characterization and inhibition analysis of the acetylcholinesterase gene from the silkworm maggot, Exorista sorbillans - Lang_2010_BMB.Rep_43_573
Author(s) : Lang GJ , Zhang MY , Li BL , Yu LL , Lu XM , Zhang CX
Ref : BMB Rep , 43 :573 , 2010
Abstract :

Several organophosphorus (OP) insecticides can selectively kill the silkworm maggot, Exorista sorbillans (Es) (Diptera: Tachinidae), while not obviously affecting the host (Bombyx mori) larvae, but the mechanism is not yet clear. In this study, the cDNA encoding an acetylcholinesterase (AChE) from the field Es was isolated. One point mutation (Gly353Ala) was identified. The Es-353G AChE and Es-353A AChE were expressed in baculovirus- insect cell system, respectively. The inhibition results showed that for eserine and Chlorpyrifos, Es-353A AChE was significantly less sensitive than Es-353G AChE. Meanwhile, comparison of the I(50) values of eserine, dichlorvos, Chlorpyrifos and omethoate of recombinant Es AChEs with its host (Bombyx mori) AChEs indicated that, both Es AChEs are more sensitive than B. mori AChEs. The results give an insight of the mechanism that some OP insecticides can selectively kills Es while without distinct effect on its host, B. mori.

PubMedSearch : Lang_2010_BMB.Rep_43_573
PubMedID: 20797321
Gene_locus related to this paper: exoso-ACHE2

Related information

Mutation G353A_exoso-ACHE2
Gene_locus G353A_exoso-ACHE2    exoso-ACHE2

Citations formats

Lang GJ, Zhang MY, Li BL, Yu LL, Lu XM, Zhang CX (2010)
Molecular characterization and inhibition analysis of the acetylcholinesterase gene from the silkworm maggot, Exorista sorbillans
BMB Rep 43 :573

Lang GJ, Zhang MY, Li BL, Yu LL, Lu XM, Zhang CX (2010)
BMB Rep 43 :573