Pheophytinase
Relationship
Comment
Pheophytin pheophorbide hydrolase (pheophytinase) is involved in chlorophyll breakdown during leaf senescence. Chlorophyllase (the other first step chrorophylle degrading enzyme) is not localized to plastids, and double knockout mutant plants still are able to degrade chlorophyll during leaf senescence. So pheophytinase is a new pathway. 
This entry represents a group of pheophytinases from plants, including PPH, a chloroplast-located and senescence-induced hydrolase widely distributed in algae and land plants. In Arabidopsis, PPH (also known as CRN1) specifically dephytylates the Mg-free chlorophyll pigment, pheophytin (phein), yielding pheophorbide. It plays a crucial role in Chlorophyll degradation during leaf senescence
Database
Interpro : IPR044211 Pheophytinase, chloroplastic PPH_chloroplastic
PIRSF : No PIRSF
Pdoc : No Pdoc
Pfam : PF12697 Abhydrolase_6
Prints : No Print
EC Number : No EC Number
Sequences
| Peptide in | Fasta |
| Nucleotide in | Fasta |
| Alignment with Multalin | Text only |
| Seed alignment with MAFFT | No colour / coloured with Mview |
| Alignment with MAFFT | No colour / coloured with Mview |
| Dendrogram | The dnd file |
| Gene_locus | Name | Species |
|---|---|---|
| 9chlo-i0yle3 | Coccomyxa subellipsoidea C-169 Alpha\/beta-hydrolase | Coccomyxa subellipsoidea C-169 |
| arath-Q9FFZ1 | Arabidopsis thaliana (Mouse-ear cress) Arabidopsis lyrata subsp. lyrata (Lyre-leaved rock-cress) PPH, non yellow coloring 3, At5g13800, Pheophytinase, chloroplastic CRN1 | Arabidopsis thaliana |
| bradi-i1gx82 | Brachypodium distachyon (Purple false brome) (Trachynia distachya) Uncharacterized protein | Brachypodium distachyon |
| brarc-g0ywa8 | Brassica rapa var. parachinensis Chromoplast alpha\/beta fold family protein | Brassica rapa var. parachinensis |
| chlre-a8jhc8 | Chlamydomonas reinhardtii (Chlamydomonas smithii) Predicted protein | Chlamydomonas reinhardtii |
| cucsa-c6l816 | Cucumis sativus (Cucumber) Putative pheophytinase | Cucumis sativus |
| ectsi-d7fwk0 | Ectocarpus siliculosus (Brown alga) Putative uncharacterized protein | Ectocarpus siliculosus |
| horvd-f2dlw1 | Hordeum vulgare var. distichum (Two-rowed barley) Predicted protein | Hordeum vulgare var. distichum |
| horvd-f2e504 | Hordeum vulgare var. distichum (Two-rowed barley)\; subsp. vulgare (Domesticated barley) Predicted protein | Hordeum vulgare var. distichum |
| maize-b4fz64 | Zea mays (Maize) Putative uncharacterized protein | Zea mays |
| maize-b6ti01 | Zea mays (Maize) Hydrolase, alpha\/beta fold family protein | Zea mays |
| medtr-g7izm0 | Medicago truncatula (Barrel medic) (Medicago tribuloides) Alpha\/beta hydrolase-fold family protein | Medicago truncatula |
| micpc-c1mie7 | Micromonas pusilla (strain CCMP1545) (Picoplanktonic green alga) Predicted protein | Micromonas pusilla |
| micsr-c1e2u5 | Micromonas sp. (strain RCC299 \/ NOUM17) (Picoplanktonic green alga) Hydrolase-like protein | Micromonas sp. |
| orysa-q69xr2 | Oryza sativa (japonica cultivar-group) Oryza glaberrima (African rice) hydrolase-like Non Yellow coloring 3 | Oryza sativa |
| ostlu-a4rrl5 | Ostreococcus lucimarinus (strain CCE9901) Predicted protein | Ostreococcus lucimarinus |
| ostta-q01g03 | Ostreococcus tauri Hydrolase-like (ISS) | Ostreococcus tauri |
| phypa-a9s5n8 | Physcomitrella patens subsp. patens (Moss) Predicted protein | Physcomitrella patens subsp. patens |
| poptr-b9hnv3 | Populus trichocarpa (Western balsam poplar) (Populus balsamifera subsp. trichocarpa) AB hydrolase-1 domain-containing protein | Populus trichocarpa |
| ricco-b9riy7 | Ricinus communis (Castor bean) Alpha\/beta hydrolase, putative | Ricinus communis |
| sorbi-c5xtz0 | Sorghum bicolor (Sorghum) (Sorghum vulgare) Putative uncharacterized protein Sb04g002990 | Sorghum bicolor |
| soybn-i1l5j6 | Glycine max (Soybean) (Glycine hispida) Uncharacterized protein | Glycine max |
| soybn-i1lkf7 | Glycine max (Soybean) (Glycine hispida)\; Glycine soja (Wild soybean) Uncharacterized protein | Glycine max |
| soybn-i1lnz2 | Glycine max (Soybean) (Glycine hispida) Uncharacterized protein | Glycine max |
| tobac-a7wpl1 | Nicotiana tabacum (Common tobacco) Putative esterase | Nicotiana tabacum |
| vitvi-f6hhx5 | Vitis vinifera (Grape) Putative uncharacterized protein | Vitis vinifera |
| volca-d8tns6 | Volvox carteri (Green alga) Putative uncharacterized protein | Volvox carteri |
| Gene_locus_frgt | Name | Species |
|---|---|---|
| 9rosa-g3k718 | Pyrus x bretschneideri Pheophytinase | Pyrus x bretschneideri |
| poptr-b9gf19 | Populus trichocarpa (Western balsam poplar) (Populus balsamifera subsp. trichocarpa) Predicted protein | Populus trichocarpa |
| soybn-c6t6h4 | Glycine max (Soybean) (Glycine hispida) Putative uncharacterized protein | Glycine max |
| Substrate | Chemical Nomenclature | Proteins |
|---|---|---|
| Chlorophyll-a | Magnesium, ((2E,7R,11R)-3,7,11,15-tetramethyl-2-hexadecenyl (3S,4S,21R)-9-ethenyl-14-ethyl-21-(methoxycarbonyl)-4,8,13,18-tetramethyl-20-oxo-3-phorbinepropanoato(2-)-kappaN23,kappaN24,kappaN25,kappaN26)-, (SP-4-2)- | arath-clh1 arath-clh2 wheat-w5h2c8 9cyan-k9tid0 chlre-a8j2s9 |
| Phaeophytin-a | methyl (3R,21S,22S)-16-ethenyl-11-ethyl-4-hydroxy-12,17,21,26-tetramethyl-22-[3-oxo-3-[(E,7R,11R)-3,7,11,15-tetramethylhexadec-2-enoxy]propyl]-7,23,24,25-tetrazahexacyclo[18.2.1.15,8.110,13.115,18.02,6]hexacosa-1,4,6,8(26),9,11,13(25),14,16,18(24),19-undecaene-3-carboxylate | arath-Q9FFZ1 wheat-w5h2c8 9cyan-k9tid0 chlre-a8j2s9 |
References (4)
| Title : Catalytic and structural properties of pheophytinase, the phytol esterase involved in chlorophyll breakdown - Guyer_2018_J.Exp.Bot_69_879 |
| Author(s) : Guyer L , Salinger K , Krugel U , Hortensteiner S |
| Ref : J Exp Bot , 69 :879 , 2018 |
| Abstract : |
| PubMedSearch : Guyer_2018_J.Exp.Bot_69_879 |
| PubMedID: 29036670 |
| Gene_locus related to this paper: arath-Q9FFZ1 |
| Title : Reverse genetic identification of CRN1 and its distinctive role in chlorophyll degradation in Arabidopsis - Ren_2010_J.Integr.Plant.Biol_52_496 |
| Author(s) : Ren G , Zhou Q , Wu S , Zhang Y , Zhang L , Huang J , Sun Z , Kuai B |
| Ref : J Integr Plant Biol , 52 :496 , 2010 |
| Abstract : |
| PubMedSearch : Ren_2010_J.Integr.Plant.Biol_52_496 |
| PubMedID: 20537045 |
| Gene_locus related to this paper: arath-Q9FFZ1 |
| Title : Pheophytin pheophorbide hydrolase (pheophytinase) is involved in chlorophyll breakdown during leaf senescence in Arabidopsis - Schelbert_2009_Plant.Cell_21_767 |
| Author(s) : Schelbert S , Aubry S , Burla B , Agne B , Kessler F , Krupinska K , Hortensteiner S |
| Ref : Plant Cell , 21 :767 , 2009 |
| Abstract : |
| PubMedSearch : Schelbert_2009_Plant.Cell_21_767 |
| PubMedID: 19304936 |
| Gene_locus related to this paper: arath-Q9FFZ1 |
| Title : A new chlorophyll degradation pathway - |
| Author(s) : Eckardt NA |
| Ref : Plant Cell , 21 :700 , 2009 |
| PubMedID: 19304932 |