Family: Pheophytinase

Block: X

Parent Family: Abhydrolase_6


Pheophytin pheophorbide hydrolase (pheophytinase) is involved in chlorophyll breakdown during leaf senescence. Chlorophyllase (the other first step chrorophylle degrading enzyme) is not localized to plastids, and double knockout mutant plants still are able to degrade chlorophyll during leaf senescence. So pheophytinase is a new pathway. This entry represents a group of pheophytinases from plants, including PPH, a chloroplast-located and senescence-induced hydrolase widely distributed in algae and land plants. In Arabidopsis, PPH (also known as CRN1) specifically dephytylates the Mg-free chlorophyll pigment, pheophytin (phein), yielding pheophorbide. It plays a crucial role in Chlorophyll degradation during leaf senescence


Interpro : IPR044211 Pheophytinase, chloroplastic PPH_chloroplastic


Pdoc : No Pdoc

Pfam : PF12697 Abhydrolase_6

Prints : No Print

EC Number : No EC Number


Peptide in Fasta
Nucleotide in Fasta
Alignment with Multalin Text only
Seed alignment with MAFFT No colour / coloured with Mview
Alignment with MAFFT No colour / coloured with Mview
Dendrogram The dnd file

Gene_locus Name Species
9chlo-i0yle3 Coccomyxa subellipsoidea C-169 Alpha\/beta-hydrolase Coccomyxa subellipsoidea C-169
arath-Q9FFZ1 Arabidopsis thaliana (Mouse-ear cress) Arabidopsis lyrata subsp. lyrata (Lyre-leaved rock-cress) PPH, non yellow coloring 3, At5g13800, Pheophytinase, chloroplastic CRN1 Arabidopsis thaliana
bradi-i1gx82 Brachypodium distachyon (Purple false brome) (Trachynia distachya) Uncharacterized protein Brachypodium distachyon
brarc-g0ywa8 Brassica rapa var. parachinensis Chromoplast alpha\/beta fold family protein Brassica rapa var. parachinensis
chlre-a8jhc8 Chlamydomonas reinhardtii (Chlamydomonas smithii) Predicted protein Chlamydomonas reinhardtii
cucsa-c6l816 Cucumis sativus (Cucumber) Putative pheophytinase Cucumis sativus
ectsi-d7fwk0 Ectocarpus siliculosus (Brown alga) Putative uncharacterized protein Ectocarpus siliculosus
horvd-f2dlw1 Hordeum vulgare var. distichum (Two-rowed barley) Predicted protein Hordeum vulgare var. distichum
horvd-f2e504 Hordeum vulgare var. distichum (Two-rowed barley)\; subsp. vulgare (Domesticated barley) Predicted protein Hordeum vulgare var. distichum
maize-b4fz64 Zea mays (Maize) Putative uncharacterized protein Zea mays
maize-b6ti01 Zea mays (Maize) Hydrolase, alpha\/beta fold family protein Zea mays
medtr-g7izm0 Medicago truncatula (Barrel medic) (Medicago tribuloides) Alpha\/beta hydrolase-fold family protein Medicago truncatula
micpc-c1mie7 Micromonas pusilla (strain CCMP1545) (Picoplanktonic green alga) Predicted protein Micromonas pusilla
micsr-c1e2u5 Micromonas sp. (strain RCC299 \/ NOUM17) (Picoplanktonic green alga) Hydrolase-like protein Micromonas sp.
orysa-q69xr2 Oryza sativa (japonica cultivar-group) Oryza glaberrima (African rice) hydrolase-like Non Yellow coloring 3 Oryza sativa
ostlu-a4rrl5 Ostreococcus lucimarinus (strain CCE9901) Predicted protein Ostreococcus lucimarinus
ostta-q01g03 Ostreococcus tauri Hydrolase-like (ISS) Ostreococcus tauri
phypa-a9s5n8 Physcomitrella patens subsp. patens (Moss) Predicted protein Physcomitrella patens subsp. patens
poptr-b9hnv3 Populus trichocarpa (Western balsam poplar) (Populus balsamifera subsp. trichocarpa) AB hydrolase-1 domain-containing protein Populus trichocarpa
ricco-b9riy7 Ricinus communis (Castor bean) Alpha\/beta hydrolase, putative Ricinus communis
sorbi-c5xtz0 Sorghum bicolor (Sorghum) (Sorghum vulgare) Putative uncharacterized protein Sb04g002990 Sorghum bicolor
soybn-i1l5j6 Glycine max (Soybean) (Glycine hispida) Uncharacterized protein Glycine max
soybn-i1lkf7 Glycine max (Soybean) (Glycine hispida)\; Glycine soja (Wild soybean) Uncharacterized protein Glycine max
soybn-i1lnz2 Glycine max (Soybean) (Glycine hispida) Uncharacterized protein Glycine max
tobac-a7wpl1 Nicotiana tabacum (Common tobacco) Putative esterase Nicotiana tabacum
vitvi-f6hhx5 Vitis vinifera (Grape) Putative uncharacterized protein Vitis vinifera
volca-d8tns6 Volvox carteri (Green alga) Putative uncharacterized protein Volvox carteri

Gene_locus_frgt Name Species
9rosa-g3k718 Pyrus x bretschneideri Pheophytinase Pyrus x bretschneideri
poptr-b9gf19 Populus trichocarpa (Western balsam poplar) (Populus balsamifera subsp. trichocarpa) Predicted protein Populus trichocarpa
soybn-c6t6h4 Glycine max (Soybean) (Glycine hispida) Putative uncharacterized protein Glycine max

Substrate Chemical Nomenclature Proteins
Phaeophytin-a methyl (3R,21S,22S)-16-ethenyl-11-ethyl-4-hydroxy-12,17,21,26-tetramethyl-22-[3-oxo-3-[(E,7R,11R)-3,7,11,15-tetramethylhexadec-2-enoxy]propyl]-7,23,24,25-tetrazahexacyclo[,8.110,13.115,18.02,6]hexacosa-1,4,6,8(26),9,11,13(25),14,16,18(24),19-undecaene-3-carboxylate arath-Q9FFZ1    wheat-w5h2c8    9cyan-k9tid0    chlre-a8j2s9
Chlorophyll-a Magnesium, ((2E,7R,11R)-3,7,11,15-tetramethyl-2-hexadecenyl (3S,4S,21R)-9-ethenyl-14-ethyl-21-(methoxycarbonyl)-4,8,13,18-tetramethyl-20-oxo-3-phorbinepropanoato(2-)-kappaN23,kappaN24,kappaN25,kappaN26)-, (SP-4-2)- arath-clh1    arath-clh2    wheat-w5h2c8    9cyan-k9tid0    chlre-a8j2s9

References (4)

Title : Catalytic and structural properties of pheophytinase, the phytol esterase involved in chlorophyll breakdown - Guyer_2018_J.Exp.Bot_69_879
Author(s) : Guyer L , Salinger K , Krugel U , Hortensteiner S
Ref : J Exp Bot , 69 :879 , 2018
Abstract :
PubMedSearch : Guyer_2018_J.Exp.Bot_69_879
PubMedID: 29036670
Gene_locus related to this paper: arath-Q9FFZ1

Title : Reverse genetic identification of CRN1 and its distinctive role in chlorophyll degradation in Arabidopsis - Ren_2010_J.Integr.Plant.Biol_52_496
Author(s) : Ren G , Zhou Q , Wu S , Zhang Y , Zhang L , Huang J , Sun Z , Kuai B
Ref : J Integr Plant Biol , 52 :496 , 2010
Abstract :
PubMedSearch : Ren_2010_J.Integr.Plant.Biol_52_496
PubMedID: 20537045
Gene_locus related to this paper: arath-Q9FFZ1

Title : A new chlorophyll degradation pathway -
Author(s) : Eckardt NA
Ref : Plant Cell , 21 :700 , 2009
PubMedID: 19304932

Title : Pheophytin pheophorbide hydrolase (pheophytinase) is involved in chlorophyll breakdown during leaf senescence in Arabidopsis - Schelbert_2009_Plant.Cell_21_767
Author(s) : Schelbert S , Aubry S , Burla B , Agne B , Kessler F , Krupinska K , Hortensteiner S
Ref : Plant Cell , 21 :767 , 2009
Abstract :
PubMedSearch : Schelbert_2009_Plant.Cell_21_767
PubMedID: 19304936
Gene_locus related to this paper: arath-Q9FFZ1