RutD is encoded within the Rut operon which allows growth on pyrimidines as sole nitrogen source. The function of this protein is not known, but it is necessary for growth on pyrimidines, and cells lacking RutD produce much less of the Rut pathway intermediate malonic semialdehyde than normal. It is thought that RutD increases the rate of spontaneous hydrolysis of the toxic intermediate aminoacrylate to malonic semialdehyde. The closest relative family is Carboxymethylbutenolide_lactonase. The structure of E. Coli RutD by Knapik et al. revealed a new active site for an alphabeta hydrolase: The nucleophile residue is Histidine located in a pentapptide GHALG. (a similar pentapeptide GHALD with alanine as nucleophile has been found in an un related family Methylketone synthase 1 in hydroxylnitrile lyase Auldridge et al.)
Interpro : IPR019913 Pyrimidine utilisation protein RutD
PIRSF : PIRSF000875 3-oxoadipate enol-lactonase
Pdoc : No Pdoc
Pfam : PF12697 Abhydrolase_6
Prints : No Print
EC Number : No EC Number
Structures (1)
Genes Proteins in RutD family (30)
No fragment of genes
Structures in RutD family (1)Substrates in RutD family (1)
No Inhibitor
Title : A multi-faceted analysis of RutD reveals a novel family of alpha\/beta hydrolases - Knapik_2012_Proteins_80_2359 |
Author(s) : Knapik AA , Petkowski JJ , Otwinowski Z , Cymborowski MT , Cooper DR , Majorek KA , Chruszcz M , Krajewska WM , Minor W |
Ref : Proteins , 80 :2359 , 2012 |
Abstract : Knapik_2012_Proteins_80_2359 |
ESTHER : Knapik_2012_Proteins_80_2359 |
PubMedSearch : Knapik_2012_Proteins_80_2359 |
PubMedID: 22641504 |
Gene_locus related to this paper: ecoli-rutD |
Title : The Rut pathway for pyrimidine degradation: novel chemistry and toxicity problems. - Kim_2010_J.Bacteriol_192_4089 |
Author(s) : Kim KS , Pelton JG , Inwood WB , Andersen U , Kustu S , Wemmer DE |
Ref : Journal of Bacteriology , 192 :4089 , 2010 |
Abstract : Kim_2010_J.Bacteriol_192_4089 |
ESTHER : Kim_2010_J.Bacteriol_192_4089 |
PubMedSearch : Kim_2010_J.Bacteriol_192_4089 |
PubMedID: 20400551 |
Gene_locus related to this paper: ecoli-rutD |
Title : A previously undescribed pathway for pyrimidine catabolism - Loh_2006_Proc.Natl.Acad.Sci.U.S.A_103_5114 |
Author(s) : Loh KD , Gyaneshwar P , Markenscoff Papadimitriou E , Fong R , Kim KS , Parales R , Zhou Z , Inwood W , Kustu S |
Ref : Proc Natl Acad Sci U S A , 103 :5114 , 2006 |
Abstract : Loh_2006_Proc.Natl.Acad.Sci.U.S.A_103_5114 |
ESTHER : Loh_2006_Proc.Natl.Acad.Sci.U.S.A_103_5114 |
PubMedSearch : Loh_2006_Proc.Natl.Acad.Sci.U.S.A_103_5114 |
PubMedID: 16540542 |
Gene_locus related to this paper: ecoli-rutD |