Loh_2006_Proc.Natl.Acad.Sci.U.S.A_103_5114

Reference

Title : A previously undescribed pathway for pyrimidine catabolism - Loh_2006_Proc.Natl.Acad.Sci.U.S.A_103_5114
Author(s) : Loh KD , Gyaneshwar P , Markenscoff Papadimitriou E , Fong R , Kim KS , Parales R , Zhou Z , Inwood W , Kustu S
Ref : Proc Natl Acad Sci U S A , 103 :5114 , 2006
Abstract :

The b1012 operon of Escherichia coli K-12, which is composed of seven unidentified ORFs, is one of the most highly expressed operons under control of nitrogen regulatory protein C. Examination of strains with lesions in this operon on Biolog Phenotype MicroArray (PM3) plates and subsequent growth tests indicated that they failed to use uridine or uracil as the sole nitrogen source and that the parental strain could use them at room temperature but not at 37 degrees C. A strain carrying an ntrB(Con) mutation, which elevates transcription of genes under nitrogen regulatory protein C control, could also grow on thymidine as the sole nitrogen source, whereas strains with lesions in the b1012 operon could not. Growth-yield experiments indicated that both nitrogens of uridine and thymidine were available. Studies with [(14)C]uridine indicated that a three-carbon waste product from the pyrimidine ring was excreted. After trimethylsilylation and gas chromatography, the waste product was identified by mass spectrometry as 3-hydroxypropionic acid. In agreement with this finding, 2-methyl-3-hydroxypropionic acid was released from thymidine. Both the number of available nitrogens and the waste products distinguished the pathway encoded by the b1012 operon from pyrimidine catabolic pathways described previously. We propose that the genes of this operon be named rutA-G for pyrimidine utilization. The product of the divergently transcribed gene, b1013, is a tetracycline repressor family regulator that controls transcription of the b1012 operon negatively.

PubMedSearch : Loh_2006_Proc.Natl.Acad.Sci.U.S.A_103_5114
PubMedID: 16540542
Gene_locus related to this paper: ecoli-rutD

Related information

Gene_locus ecoli-rutD
Family ecoli-rutD    RutD

Citations formats

Loh KD, Gyaneshwar P, Markenscoff Papadimitriou E, Fong R, Kim KS, Parales R, Zhou Z, Inwood W, Kustu S (2006)
A previously undescribed pathway for pyrimidine catabolism
Proc Natl Acad Sci U S A 103 :5114

Loh KD, Gyaneshwar P, Markenscoff Papadimitriou E, Fong R, Kim KS, Parales R, Zhou Z, Inwood W, Kustu S (2006)
Proc Natl Acad Sci U S A 103 :5114