Thioesterase_acyl-transferase
Relationship
Comment
This bacterial family of Acyl transferases (ACT or myristoyl-acp-specific thioesterases) catalyses the first step in the bioluminescent fatty acid reductase system, which is required for aldehyde biosynthesis. This enzyme belongs to the LuxD family. Together with acyl-protein synthetase (LuxE) and reductase (LuxC), it belongs to a multienzyme complex. This complex channels activated fatty acids into the aldehyde substrate for the luciferase-catalyzed bacterial bioluminescence reaction. The C-terminal region of LuxD interacts with LuxE to causes a conformational change. LuxD has a calculated M(r) of 34,384 and comprises 305 aa residues. Induction of luminescence only occurs at high cell density. Some bacteria have N-acylhomoserine lactone autoinducers for luminescence. Warning: the serine 77 in GXSXG motif is not the active site serine 114 as determined by X ray structure AASLS in Vibrio harveyi see Lawson et al., 2003. Family TE19 in ThYme database
Database
Interpro : IPR003157 Acyl transferase, LuxD
PIRSF : PIRSF009416 lux-specific fatty acid reductase, acyl-ACP thioesterase subunit
Pdoc : No Pdoc
Pfam : PF02273 Acyl_transf_2
Prints : No Print
EC Number : No EC Number
Sequences
| Peptide in | Fasta |
| Nucleotide in | Fasta |
| Alignment with Multalin | Text only |
| Seed alignment with MAFFT | No colour / coloured with Mview |
| Alignment with MAFFT | No colour / coloured with Mview |
| Dendrogram | The dnd file |
| Structure | Name | Proteins |
|---|---|---|
| 1THT | Vibrio harveyi Myristoyl-ACP-specific thioesterase | vibha-1luxd |
| Gene_locus | Name | Species |
|---|---|---|
| 9bact-d8pbw6 | Candidatus Nitrospira defluvii Putative uncharacterized protein | Candidatus Nitrospira defluvii |
| 9bact-d8pdl3 | Candidatus Nitrospira defluvii Putative uncharacterized protein | Candidatus Nitrospira defluvii |
| 9delt-a6g1j0 | Plesiocystis pacifica SIR-1 LuxD. acyl transferase | Plesiocystis pacifica SIR-1 |
| 9gamm-q4a538 | Vibrio salmonicida Aliivibrio salmonicida (strain LFI1238) (Vibrio salmonicida (strain LFI1238)) luxd | Vibrio salmonicida |
| corad-d5ene2 | Coraliomargarita akajimensis (strain DSM 45221 \/ IAM 15411 \/ JCM 2319 \/ KCTC 12865) Methyltransferase type 11 | Coraliomargarita akajimensis |
| phole-lxd1 | Photobacterium leiognathi subsp. leiognathi Photobacterium mondopomensis Photobacterium damselae thioesterase (c14acp-te) | Photobacterium leiognathi |
| phole-lxd2 | Photobacterium leiognathi Photobacterium mondopomensis thioesterase (c14acp-te) | Photobacterium leiognathi |
| pholu-LUXD2 | Photorhabdus luminescens (Xenorhabdus luminescens) and (subsp. laumondii) acyl transferase | Photorhabdus luminescens |
| pholu-lxd1 | Photorhabdus luminescens (Xenorhabdus luminescens) thioesterase | Photorhabdus luminescens |
| pholu-lxd2 | Photorhabdus luminescens (Xenorhabdus luminescens), Photorhabdus asymbiotica, thioesterase (c14acp-te) | Photorhabdus luminescens |
| phopo-luxd | Photobacterium phosphoreum Photobacterium kishitanii, thioesterase | Photobacterium phosphoreum |
| sheha-LUXD | Shewanella hanedai (Alteromonas hanedai) myristoryl-acp thioesterase (c14acp-te) | Shewanella hanedai |
| shewm-luxd | Shewanella woodyi (and strain ATCC 51908 \/ MS32) Myristoyl-ACP-specific thioesterase | Shewanella woodyi |
| vibcl-q5ek34 | Vibrio cholerae (strains bv. albensis\; VL426\; RC385) Vibrio sp. RC586 putative acyltransferase (fragment) luxD | Vibrio cholerae |
| vibf1-q5dz04 | Vibrio fischeri (strain ATCC 700601 \/ ES114) luxd. acyl transferase (EC 2.3.1.-) | Vibrio fischeri |
| vibfi-LUXD | Vibrio fischeri (and strain MJ11) acyl-transferase luxd (c14acp-te) | Vibrio fischeri |
| vibha-1luxd |  Vibrio harveyi, Vibrio orientalis, Vibrio vulnificus, Vibrio chagasii myristoyl-ACP-specific thioesterase acyl transferase (luxD) gene |
Vibrio harveyi |
| Substrate | Chemical Nomenclature | Proteins |
|---|---|---|
| Myristoyl-ACP | Myristoyl-Acyl-carrier protein |
References (5)
| Title : Thioesterase enzyme families: Functions, structures, and mechanisms - Caswell_2021_Protein.Sci__ |
| Author(s) : Caswell BT , de Carvalho CC , Nguyen H , Roy M , Nguyen T , Cantu DC |
| Ref : Protein Science , : , 2021 |
| Abstract : |
| PubMedSearch : Caswell_2021_Protein.Sci__ |
| PubMedID: 34921469 |
| Title : ThYme: a database for thioester-active enzymes - Cantu_2011_Nucleic.Acids.Res_39_D342 |
| Author(s) : Cantu DC , Chen Y , Lemons ML , Reilly PJ |
| Ref : Nucleic Acids Research , 39 :D342 , 2011 |
| Abstract : |
| PubMedSearch : Cantu_2011_Nucleic.Acids.Res_39_D342 |
| PubMedID: 21045059 |
| Title : Thioesterases: a new perspective based on their primary and tertiary structures. - Cantu_2010_Protein.Sci_19_1281 |
| Author(s) : Cantu DC , Chen Y , Reilly PJ |
| Ref : Protein Science , 19 :1281 , 2010 |
| Abstract : |
| PubMedSearch : Cantu_2010_Protein.Sci_19_1281 |
| PubMedID: 20506386 |
| Title : Structure of a myristoyl-ACP-specific thioesterase from Vibrio harveyi - Lawson_1994_Biochemistry_33_9382 |
| Author(s) : Lawson DM , Derewenda U , Serre L , Ferri S , Szittner R , Wei Y , Meighen EA , Derewenda ZS |
| Ref : Biochemistry , 33 :9382 , 1994 |
| Abstract : |
| PubMedSearch : Lawson_1994_Biochemistry_33_9382 |
| PubMedID: 8068614 |
| Gene_locus related to this paper: pholu-lxd1 , phopo-luxd , vibha-1luxd |
| Title : Sequence of the luxD gene encoding acyltransferase of the lux operon from Photobacterium leiognathi - Chao_1993_Gene_126_155 |
| Author(s) : Chao YF , Weng SF , Lin JW |
| Ref : Gene , 126 :155 , 1993 |
| Abstract : |
| PubMedSearch : Chao_1993_Gene_126_155 |
| PubMedID: 8472957 |
| Gene_locus related to this paper: phole-lxd2 |