uncultured bacterium EST5
Optimum pH 7.5, 45 C, with a Tm of 47 C. The enzyme remained at least 50\% active at low temperatures and exhibited broad substrate specificity toward p-nitrophenol esters. EST5 remained stable in the presence of up to 10\% methanol and 20\% dimethyl sulfoxide
Family : Aclacinomycin-methylesterase_RdmC
Block : X
Position in NCBI Life Tree : uncultured bacterium
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)
> cellular organisms N E > Bacteria N E > environmental samples N E > uncultured bacterium N E > cellular organisms N E > Bacteria N E > environmental samples N E > uncultured bacterium N E > cellular organisms N E > Bacteria N E > environmental samples N E > uncultured bacterium N EMTVNMKTAKA NGITINYEDR GPADGTPLLF VNGYTSTMMN WPSELMNAFK ARGFRVIRYD NRDVGLSEKL KGVPKIDEVV AALRAGETPR IPYTLADMAA DGIGLLDALG IKRAHVMGIS MGGMIVQSMA INHPDRLLSV TSIMSTTGNF DLPKATDEAM AALQQVPASD EREAVIRHRM KGRRVYQSPA YPMTDEALYA RCAAEFDHMY YPEGASRQYA AIVGDGSRVE RLKKVHVPFL VIHGKADPLV RVEGGIDTAK SVPGARLELI DGMGHDMPVE LTSRFVELIE AHARAAERKA AAE
| Title : Exploring Metagenomic Enzymes: A Novel Esterase Useful for Short-Chain Ester Synthesis - Maester_2020_Catalysts_10_1100 |
| Author(s) : Maester TC , Pereira MR , Malaman AMG , Borges JP , Pereira PAM , Lemos EGM |
| Ref : Catalysts , 10 :1100 , 2020 |
| Abstract : |
| PubMedSearch : Maester_2020_Catalysts_10_1100 |
| PubMedID: |
| Gene_locus related to this paper: 9bact-EST5 |
| Title : Exploring Metagenomic Enzymes: A Novel Esterase Useful for Short-Chain Ester Synthesis - Maester_2020_Catalysts_10_1100 |
| Author(s) : Maester TC , Pereira MR , Malaman AMG , Borges JP , Pereira PAM , Lemos EGM |
| Ref : Catalysts , 10 :1100 , 2020 |
| Abstract : |
| PubMedSearch : Maester_2020_Catalysts_10_1100 |
| PubMedID: |
| Gene_locus related to this paper: 9bact-EST5 |
| Title : Exploring Metagenomic Enzymes: A Novel Esterase Useful for Short-Chain Ester Synthesis - Maester_2020_Catalysts_10_1100 |
| Author(s) : Maester TC , Pereira MR , Malaman AMG , Borges JP , Pereira PAM , Lemos EGM |
| Ref : Catalysts , 10 :1100 , 2020 |
| Abstract : |
| PubMedSearch : Maester_2020_Catalysts_10_1100 |
| PubMedID: |
| Gene_locus related to this paper: 9bact-EST5 |