Thermocarpiscus australiensis cutinase TaC
PET hydrolase four times higher activity than that of Humiclo insolens HiC, but required high salt concentrations (about 0.5 M NaCl) to reach maximal activity. Someariants with a reduced (negative) net charge, performed up to 2.5-fold better in salt-free buffer, and some escaped the dependence of salt but retained the maximal catalytic performance of the wild type.
Family : Cutinase\
Block : X
Position in NCBI Life Tree : Thermocarpiscus australiensis
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)
MKFLQIFSLA GLVTALPTLP TEDAVEIEAR QVSSTSNDLE NGSSANCPRV ILVFARGSTE PGNMGATVGP DLANGLATYF PNDLWVQGVG GAYDATLEAN ALPLGTTQAA IDEAARLFTL ANQKCPSSAV VAGGYSQGTA VIAGALSGLA GSAVQDQVRG AALFGYTRNA QNGGRIPNFP TDRTQVYCNP GDLVCTGTLI VTPAHFGYGV DASVTAPRWL AGQVNGA
| Title : Discovery and Surface Charge Engineering of Fungal Cutinases for Enhanced Activity on Poly(ethylene terephthalate) - Brinch-Pedersen_2024_ACS.Sustainable.Chem.Eng_12_7329 |
| Author(s) : Brinch-Pedersen W , Billeskov Keller M , Dorau R , Paul B , Jensen K , Borch K , Westh P |
| Ref : ACS Sustainable Chem. Eng. , 12 :7329 , 2024 |
| Abstract : |
| PubMedSearch : Brinch-Pedersen_2024_ACS.Sustainable.Chem.Eng_12_7329 |
| PubMedID: |
| Gene_locus related to this paper: 9pezi-TaCutinase , 9pezi-RtCutinase , 9pezi-DmCutinase |