Cutinase

Relationship

Family: Cutinase

Block: X

Parent Family: Cutinase_like

Comment

This family corresponds to the Carbohydrate Esterase family CE5 in CAZy - Carbohydrate-Active enZYmes database (CE_5). (from interpro) Aerial plant organs are protected by a cuticle composed of an insoluble polymeric structural compound, cutin, which is a polyester composed of hydroxy and hydroxyepoxy fatty acids. Plant pathogenic fungi produce extracellular degradative enzymes that play an important role in pathogenesis. They include cutinase, which hydrolyses cutin, facilitating fungus penetration through the cuticle. Inhibition of the enzyme can prevent fungal infection through intact cuticles. Cutin monomers released from the cuticle by small amounts of cutinase on fungal spore surfaces can greatly increase the amount of cutinase secreted by the spore, the mechanism for which process is as yet unknown. It is shown by Masaki et al. that some Cutinase-Like enzyme hydrolyzes polylactic acid and other biodegradable plastics. This family differs substantially from the Polyesterase-lipase-cutinase family. Here bacterial and fungal enzymes important in plant pathogenicity

Database

Interpro : IPR011150 Cutinase, monofunctional Cutinase_monf , IPR000675 Cutinase\/acetylxylan esterase Cutinase\/axe

PIRSF : No PIRSF

Pdoc : PDOC00140

Pfam : PF01083 Cutinase

Prints : PR00129

EC Number : 3.1.1.74

Sequences

Peptide in Fasta
Nucleotide in Fasta
Alignment with Multalin Text only
Seed alignment with MAFFT No colour
Alignment with MAFFT No colour
Dendrogram The dnd file

Structures (66)

Genes Proteins in Cutinase family (563)

Fragments of genes in Cutinase family (16)

Structures in Cutinase family (66)

Substrates in Cutinase family (33)

Inhibitors in Cutinase family (34)

References (18)

Title : Structural and functional studies of a Fusarium oxysporum cutinase with polyethylene terephthalate modification potential - Dimarogona_2015_Biochim.Biophys.Acta_1850_2308
Author(s) : Dimarogona M , Nikolaivits E , Kanelli M , Christakopoulos P , Sandgren M , Topakas E
Ref : Biochimica & Biophysica Acta , 1850 :2308 , 2015
PubMedID: 26291558
Gene_locus related to this paper: fusox-CUT

Title : A Cutinase from Trichoderma reesei with a Lid-Covered Active Site and Kinetic Properties of True Lipases - Roussel_2014_J.Mol.Biol_426_3757
Author(s) : Roussel A , Amara S , Nyyssola A , Mateos-Diaz E , Blangy S , Kontkanen H , Westerholm-Parvinen A , Carriere F , Cambillau C
Ref : Journal of Molecular Biology , 426 :3757 , 2014
PubMedID: 25219509
Gene_locus related to this paper: hypjq-g0rh85

Title : Thermodynamic and structural investigation of the specific SDS binding of humicola insolens cutinase - Kold_2014_Protein.Sci_23_1023
Author(s) : Kold D , Dauter Z , Laustsen AK , Brzozowski AM , Turkenburg JP , Nielsen AD , Koldso H , Petersen E , Schiott B , De Maria L , Wilson KS , Svendsen A , Wimmer R
Ref : Protein Science , 23 :1023 , 2014
PubMedID: 24832484
Gene_locus related to this paper: humin-cut

Title : Tetrahydrolipstatin inhibition, functional analyses, and three-dimensional structure of a lipase essential for mycobacterial viability - Crellin_2010_J.Biol.Chem_285_30050
Author(s) : Crellin PK , Vivian JP , Scoble J , Chow FM , West NP , Brammananth R , Proellocks NI , Shahine A , Le Nours J , Wilce MC , Britton WJ , Coppel RL , Rossjohn J , Beddoe T
Ref : Journal of Biological Chemistry , 285 :30050 , 2010
PubMedID: 20656688
Gene_locus related to this paper: mycs2-a0r619

Title : Crystal structure and enhanced activity of a cutinase-like enzyme from Cryptococcus sp. strain S-2 - Kodama_2009_Proteins_77_710
Author(s) : Kodama Y , Masaki K , Kondo H , Suzuki M , Tsuda S , Nagura T , Shimba N , Suzuki E , Iefuji H
Ref : Proteins , 77 :710 , 2009
PubMedID: 19544571
Gene_locus related to this paper: crysp-Q874E9

Title : Mycobacteriophage Lysin B is a novel mycolylarabinogalactan esterase - Payne_2009_Mol.Microbiol_73_367
Author(s) : Payne K , Sun Q , Sacchettini J , Hatfull GF
Ref : Molecular Microbiology , 73 :367 , 2009
PubMedID: 19555454
Gene_locus related to this paper: bpmd2-vg12

Title : Catalysis by Glomerella cingulata cutinase requires conformational cycling between the active and inactive states of its catalytic triad - Nyon_2009_J.Mol.Biol_385_226
Author(s) : Nyon MP , Rice DW , Berrisford JM , Hounslow AM , Moir AJ , Huang H , Nathan S , Mahadi NM , Bakar FD , Craven CJ
Ref : Journal of Molecular Biology , 385 :226 , 2009
PubMedID: 18983850
Gene_locus related to this paper: colgl-cutas

Title : Solid-state structural characterization of cutinase-ECE-pincer-metal hybrids - Rutten_2009_Chemistry_15_4270
Author(s) : Rutten L , Wieczorek B , Mannie JP , Kruithof CA , Dijkstra HP , Egmond MR , Lutz M , Klein Gebbink RJ , Gros P , van Koten G
Ref : Chemistry , 15 :4270 , 2009
PubMedID: 19219875
Gene_locus related to this paper: fusso-cutas

Title : Structural and functional studies of Aspergillus oryzae cutinase: enhanced thermostability and hydrolytic activity of synthetic ester and polyester degradation - Liu_2009_J.Am.Chem.Soc_131_15711
Author(s) : Liu Z , Gosser Y , Baker PJ , Ravee Y , Lu Z , Alemu G , Li H , Butterfoss GL , Kong XP , Gross R , Montclare JK
Ref : Journal of the American Chemical Society , 131 :15711 , 2009
PubMedID: 19810726
Gene_locus related to this paper: aspor-cutas

Title : Cutinase-like enzyme from the yeast Cryptococcus sp. strain S-2 hydrolyzes polylactic acid and other biodegradable plastics - Masaki_2005_Appl.Environ.Microbiol_71_7548
Author(s) : Masaki K , Kamini NR , Ikeda H , Iefuji H
Ref : Applied Environmental Microbiology , 71 :7548 , 2005
PubMedID: 16269800
Gene_locus related to this paper: crysp-Q874E9

Title : Lipase active-site-directed anchoring of organometallics: metallopincer\/protein hybrids - Kruithof_2005_Chemistry_11_6869
Author(s) : Kruithof CA , Casado MA , Guillena G , Egmond MR , van der Kerk-van Hoof A , Heck AJ , Klein Gebbink RJ , van Koten G
Ref : Chemistry , 11 :6869 , 2005
PubMedID: 16224766
Gene_locus related to this paper: fusso-cutas

Title : Genome structure of mycobacteriophage D29: implications for phage evolution - Ford_1998_J.Mol.Biol_279_143
Author(s) : Ford ME , Sarkis GJ , Belanger AE , Hendrix RW , Hatfull GF
Ref : Journal of Molecular Biology , 279 :143 , 1998
PubMedID: 9636706
Gene_locus related to this paper: bpmd2-vg12 , bpmdd-prxh

Title : Crystal structure of cutinase covalently inhibited by a triglyceride analogue - Longhi_1997_Protein.Sci_6_275
Author(s) : Longhi S , Mannesse M , Verheij HM , De Haas GH , Egmond M , Knoops-Mouthuy E , Cambillau C
Ref : Protein Science , 6 :275 , 1997
PubMedID: 9041628
Gene_locus related to this paper: fusso-cutas

Title : Dynamics of Fusarium solani cutinase investigated through structural comparison among different crystal forms of its variants - Longhi_1996_Proteins_26_442
Author(s) : Longhi S , Nicolas A , Creveld L , Egmond M , Verrips CT , de Vlieg J , Martinez C , Cambillau C
Ref : Proteins , 26 :442 , 1996
PubMedID: 8990497
Gene_locus related to this paper: fusso-cutas

Title : Cutinase, a lipolytic enzyme with a preformed oxyanion hole - Martinez_1994_Biochemistry_33_83
Author(s) : Martinez C , Nicolas A , van Tilbeurgh H , Egloff MP , Cudrey C , Verger R , Cambillau C
Ref : Biochemistry , 33 :83 , 1994
PubMedID: 8286366
Gene_locus related to this paper: fusso-cutas , orysa-LPL1

Title : Fusarium solani cutinase is a lipolytic enzyme with a catalytic serine accessible to solvent - Martinez_1992_Nature_356_615
Author(s) : Martinez C , de Geus P , Lauwereys M , Matthyssens G , Cambillau C
Ref : Nature , 356 :615 , 1992
PubMedID: 1560844
Gene_locus related to this paper: fusso-cutas , orysa-LPL1

Title : Cloning and analysis of CUT1, a cutinase gene from Magnaporthe grisea - Sweigard_1992_Mol.Gen.Genet_232_174
Author(s) : Sweigard JA , Chumley FG , Valent B
Ref : Molecular & General Genetics , 232 :174 , 1992
PubMedID: 1557023
Gene_locus related to this paper: maggr-cutas

Title : Structure of cutinase gene, cDNA, and the derived amino acid sequence from phytopathogenic fungi - Ettinger_1987_Biochemistry_26_7883
Author(s) : Ettinger WF , Thukral SK , Kolattukudy PE
Ref : Biochemistry , 26 :7883 , 1987
PubMedID:
Gene_locus related to this paper: colca-cutas , colgl-cutas