(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Bacteria: NE > Terrabacteria group: NE > Actinobacteria [phylum]: NE > Actinobacteria [class]: NE > Streptomycetales: NE > Streptomycetaceae: NE > Streptomyces: NE > Streptomyces sp. Tu6071: NE
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MGAATDGARQGADEESPTHVTTTTRTLDVPGARLCYDVRGHGPLLLMIGS PMGSRGFTALATLLAADFTVVSYDPRGILRSTLSDPEQPAPPELVADDVR RLIAELDAGPAHVFGNSGGATTGLALLARHPDAVRSLVTHEPPVTELLPD ARQVRAAIDDLCEDYAAGRRDVALRKYSALSGVGFASPPPAGQASTRALY TPPKDVRAILDRFFGRILQPTTRYQPDVDALRTASVPVVVAGGTTSRGQL YHRTAAALAELLDTPLAAFPAGHTGFQEHPEPFADLLREAFAPHRHTRME RNGTQLPGQPPVGAHTEPLDH
Phenalinolactones are terpene glycosides with antibacterial activity. A striking structural feature is a highly oxidized gamma-butyrolactone of elusive biosynthetic origin. To investigate the genetic basis of the phenalinolactones biosynthesis, we cloned and sequenced the corresponding gene cluster from the producer strain Streptomyces sp. Tu6071. Spanning a 42 kbp region, 35 candidate genes could be assigned to putatively encode biosynthetic, regulatory, and resistance-conferring functions. Targeted gene inactivations were carried out to specifically manipulate the phenalinolactones pathway. The inactivation of a sugar methyltransferase gene and a cytochrome P450 monoxygenase gene led to the production of modified phenalinolactone derivatives. The inactivation of a Fe(II)/alpha-ketoglutarate-dependent dioxygenase gene disrupted the biosynthetic pathway within gamma-butyrolactone formation. The structure elucidation of the accumulating intermediate indicated that pyruvate is the biosynthetic precursor of the gamma butyrolactone moiety.
