Gene_Locus Report

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Gene_locus Report for: 9bace-b3c9d0.2

Bacteroides intestinalis DSM 17393 BiFae1B Putative uncharacterized protein

Comment
two gene fragments concatened Pfam A Esterase 61 282 Pfam A Esterase 421 645 Although the total sequence is really the esterase. In ESTHER only the second part which is complete is considered. The N-term is considered as a fragment


Relationship
Family|A85-Feruloyl-Esterase
Block| X
Position in NCBI Life Tree|Bacteroides intestinalis DSM 17393
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)
> cellular organisms: N E > Bacteria: N E > FCB group: N E > Bacteroidetes/Chlorobi group: N E > Bacteroidetes: N E > Bacteroidia: N E > Bacteroidales: N E > Bacteroidaceae: N E > Bacteroides: N E > Bacteroides intestinalis: N E > Bacteroides intestinalis DSM 17393: N E


Molecular evidence
Database
No mutation
No structure
No kinetic





2 substrates: Methyl-ferulate, Paranitrophenylacetate
No inhibitor
1 Genbank : ABJL02000007
1 UniProt : B3C9D0
1 UniProt : B3C9D0
1 Interpro : B3C9D0
1 Prodom : B3C9D0
1 Pfam : B3C9D0
1 PIRSF : B3C9D0
1 SUPERFAM : B3C9D0
Sequence
Graphical view for this peptide sequence: 9bace-b3c9d0.2
Colored MSA for A85-Feruloyl-Esterase (raw)
LHGARAESNVRNSKYPQLTADHRAIFRIKAPDAQKVQIDLGRKYDMVRQE
GGIWETVTDSLGEGFHYYSLLIDGVAVSDPGSETFYGMGRMASGIEVPFK
GGEYYALKDVPHGDIRMKRYYSEVSRSWRRFFVYTPAGYDTRTAEKYPVL
YLLHGGGEDERGWATQGKTDLIIDNLIAEGKAKPMLIVMLDGNMASAGFN
AGALKAFEAELMQAVIPFVEDNYRVRKEADSRALAGLSMGGIQTLYAGLY
HTDLFAHLGVFSSGWLPFYQKIADGQYEFINKNKAAIDSQLRLLWIAMGG
KEDIAYQNCQNMLKEFDTLKLKYTYSEYPGGHTWPVWRNNLYNFAQLLFN
K
Legend This sequence has been compared to family alignement (MSA)
red => minority aminoacid
blue => majority aminoacid
color intensity => conservation rate
title => sequence position(MSA position)aminoacid rate
Catalytic site
Catalytic site in the MSA

LHGARAESNVRNSKYPQLTADHRAIFRIKAPDAQKVQIDLGRKYDMVRQE
GGIWETVTDSLGEGFHYYSLLIDGVAVSDPGSETFYGMGRMASGIEVPFK
GGEYYALKDVPHGDIRMKRYYSEVSRSWRRFFVYTPAGYDTRTAEKYPVL
YLLHGGGEDERGWATQGKTDLIIDNLIAEGKAKPMLIVMLDGNMASAGFN
AGALKAFEAELMQAVIPFVEDNYRVRKEADSRALAGLSMGGIQTLYAGLY
HTDLFAHLGVFSSGWLPFYQKIADGQYEFINKNKAAIDSQLRLLWIAMGG
KEDIAYQNCQNMLKEFDTLKLKYTYSEYPGGHTWPVWRNNLYNFAQLLFN
K


Reference
    Title: Biochemical and Structural Analyses of Two Cryptic Esterases in Bacteroides intestinalis and their Synergistic Activities with Cognate Xylanases
    Wefers D, Cavalcante JJV, Schendel RR, Deveryshetty J, Wang K, Wawrzak Z, Mackie RI, Koropatkin NM, Cann I
    Ref: Journal of Molecular Biology, 429:2509, 2017 : PubMed

            


Other Papers


Send your questions or comments to :
Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
For technical information about these pages see:
ESTHER Home Page and ACEDB Home Page
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