Probable alcohol acetyltransferase that uses acetyl-CoA to synthesize acetate esters from various alcohols (Probable). Not involved in the synthesis of ethyl acetate
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) > cellular organisms: NE > Eukaryota: NE > Opisthokonta: NE > Fungi: NE > Dikarya: NE > Ascomycota: NE > saccharomyceta: NE > Saccharomycotina: NE > Saccharomycetes: NE > Saccharomycetales: NE > Phaffomycetaceae: NE > Cyberlindnera: NE > Cyberlindnera fabianii: NE
LegendThis sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA MRSTSLLMQAKAAIPDIKQLPTKHKVSLAYTIHWPHKTVNPNINIKPREP VIFLHGVFGSKKNYRDYCQTIANQNYTPVYSLDFRNHGESEHAFPLVNYS TLTQDVVDFIHEHKLEKVDIVGYSLGAKVALLTLLKHPELCRSGVIIGNA PIKTPQVKVYLKAFIKALKALGDKRPEIKSNDKAWRAKARDVMRKYIPDG DILHYLLRNIDIRKPKNITEYKPGTINFSMPISHFDNKVVEDIADWPEEE VEGLKFEGPVRVIRGTQSVFINDKGLAAYQKHFPNYTLTNFNSNHLIWAE RPLQVTKVVSDFLKKTRLLEMDGSAKALEESPKESYSRPPAHQQPLHKND FTHIDVGALPVNGAQAESTQA
Ethyl acetate is an industrially relevant ester that is currently produced exclusively through unsustainable processes. Many yeasts are able to produce ethyl acetate, but the main responsible enzyme has remained elusive, hampering the engineering of novel production strains. Here we describe the discovery of a new enzyme (Eat1) from the yeast Wickerhamomyces anomalus that resulted in high ethyl acetate production when expressed in Saccharomyces cerevisiae and Escherichia coli. Purified Eat1 showed alcohol acetyltransferase activity with ethanol and acetyl-CoA. Homologs of eat1 are responsible for most ethyl acetate synthesis in known ethyl acetate-producing yeasts, including S. cerevisiae, and are only distantly related to known alcohol acetyltransferases. Eat1 is therefore proposed to compose a novel alcohol acetyltransferase family within the alpha/beta hydrolase superfamily. The discovery of this novel enzyme family is a crucial step towards the development of biobased ethyl acetate production and will also help in selecting improved S. cerevisiae brewing strains.
The yeast Cyberlindnera fabianii is used in wastewater treatment, fermentation of alcoholic beverages, and has caused blood infections. To assist in the accurate identification of this species, and to determine the genetic basis for properties involved in fermentation and water treatment, we sequenced and annotated the genome of C. fabianii (YJS4271).
