DTNB
This chemiluminescent probe is not substrate but react with a product of reaction of a substrate hydrolyzed by an enzyme: this compound reacts with thiocholine after hydrolysis of acetylthiocholine by cholinesterases. A standard reagent for the determination of reactive sulfhydryl groups by absorbance measurements. It is used primarily for the determination of sulfhydryl and disulfide groups in proteins. The color produced is due to the formation of a thio anion, 3-carboxyl-4-nitrothiophenolate. As it reacts with sulfhydryl it can fix Cysteine and inhibit enzymes (for exemple the c term cysteine of Thioesterase II (not in the catalytic triad) Witkowska et al. 1990
General
Type : Chemiluminescent Probe, Sulfur Compound
Chemical_Nomenclature : 5-[(3-carboxy-4-nitrophenyl)disulfanyl]-2-nitrobenzoic acid
Canonical SMILES : C1=CC(=C(C=C1SSC2=CC(=C(C=C2)[N+](=O)[O-])C(=O)O)C(=O)O)[N+](=O)[O-]
InChI : InChI=1S\/C14H8N2O8S2\/c17-13(18)9-5-7(1-3-11(9)15(21)22)25-26-8-2-4-12(16(23)24)10(6-8)14(19)20\/h1-6H,(H,17,18)(H,19,20)
InChIKey : KIUMMUBSPKGMOY-UHFFFAOYSA-N
Other name(s) : 5,5'-Dithiobis(2-nitrobenzoic acid) || Ellman's Reagent || 3-Carboxy-4-nitrophenyl disulfide || CHEMBL395814 || CHEBI:86228 || SCHEMBL26775 || ZINC2041301
MW : 396.4
Formula : C14H8N2O8S2
CAS_number : 69-78-3
PubChem : 6254
UniChem : KIUMMUBSPKGMOY-UHFFFAOYSA-N
Target
Families : DTNB ligand of proteins in family
Thioesterase
Protein :
human-OLAH
ratno-sast
References (6)
| Title : The carboxyl-terminal region of thioesterase II participates in the interaction with fatty acid synthase. Use of electrospray ionization mass spectrometry to identify a carboxyl-terminally truncated form of the enzyme - Witkowska_1990_J.Biol.Chem_265_5662 |
| Author(s) : Witkowska HE , Green BN , Smith S |
| Ref : Journal of Biological Chemistry , 265 :5662 , 1990 |
| Abstract : |
| PubMedSearch : Witkowska_1990_J.Biol.Chem_265_5662 |
| PubMedID: 2318831 |
| Title : Sulfhydryl reactivity in the central nervous system: effects of electro-shock - |
| Author(s) : Ellman GL , Sullivan CV |
| Ref : Experimental Neurology , 13 :191 , 1965 |
| PubMedID: 5838162 |
| Title : A new and rapid colorimetric determination of acetylcholinesterase activity - Ellman_1961_Biochem.Pharmacol_7_88 |
| Author(s) : Ellman GL , Courtney KD , Andres V , Featherstone RM , Andres V, Jr. |
| Ref : Biochemical Pharmacology , 7 :88 , 1961 |
| Abstract : |
| PubMedSearch : Ellman_1961_Biochem.Pharmacol_7_88 |
| PubMedID: 13726518 |