H287C_human-ACHE

General

Gene Locus : human-ACHE

Mode of mutation : Site directed mutagenesis

Disease :

Summary : Omega loop Omega loop conformation test incorporation of the fluorophore acrylodan Boyd_2000_J.Biol.Chem_275_22401 Shi_2001_J.Biol.Chem_276_42196

AAA Change :

Allelic Variant :

Risk Factor :

Inhibitor :

Structure :

Disease by interaction :

Interact Gene Locus :

Xenobiotic sensitivity :

Modification : Omega loop

Torpedo_number : 280

Kinetic Parameter : No kinetic parameter

News : No news

Comment :
p.H287C His287Cys (p.H318C His318Cys in primary sequence with 31 amino-acids signal peptide) Johnson et al. mutated near the rim of the P-site H287C. Compounds were synthesized with a highly reactive methanethiosulfonyl substituent and linked to this cysteine through a disulfide bond

References (5)

Title : Tethering of ligands near the active site of acetylcholinesterase mutant H287C: Progress on a new strategy for protection against organophosphate inactivation -
Author(s) : Cusack B , Johnson JL , Hughes TF , McCullough EH , Fauq A , Romanovskis PV , Spatola AF , Rosenberry TL
Ref : In: Cholinesterases in the Second Millennium: Biomolecular and Pathological Aspects , (Inestrosa NC, Campos EO) P. Universidad Catolica de Chile-FONDAP Biomedicina :259 , 2004
PubMedID:

Title : Inhibitors tethered near the acetylcholinesterase active site serve as molecular rulers of the peripheral and acylation sites - Johnson_2003_J.Biol.Chem_278_38948
Author(s) : Johnson JL , Cusack B , Hughes TF , McCullough EH , Fauq A , Romanovskis P , Spatola AF , Rosenberry TL
Ref : Journal of Biological Chemistry , 278 :38948 , 2003
PubMedID: 12851386

Title : Inhibitors of different structure induce distinguishing conformations in the omega loop, Cys 69- Cys 96, of mouse acetylcholinesterase - Shi_2002_J.Biol.Chem_277_43301
Author(s) : Shi J , Radic Z , Taylor P
Ref : Journal of Biological Chemistry , 277 :43301 , 2002
PubMedID: 12196517

Title : Reversibly bound and covalently attached ligands induce conformational changes in the Omega loop, Cys 69-Cys 96, of mouse acetylcholinesterase - Shi_2001_J.Biol.Chem_276_42196
Author(s) : Shi J , Boyd AE , Radic Z , Taylor P
Ref : Journal of Biological Chemistry , 276 :42196 , 2001
PubMedID: 11517229

Title : Probing the active center Gorge of acetylcholinesterase by fluorophores Linked to substituted cysteines - Boyd_2000_J.Biol.Chem_275_22401
Author(s) : Boyd AE , Marnett AB , Wong L , Taylor P
Ref : Journal of Biological Chemistry , 275 :22401 , 2000
PubMedID: 10779503