Aamand_1994_Mol.Gen.Genet_245_556

Reference

Title : Chaperone-mediated activation in vivo of a Pseudomonas cepacia lipase - Aamand_1994_Mol.Gen.Genet_245_556
Author(s) : Aamand JL , Hobson AH , Buckley CM , Jorgensen ST , Diderichsen B , McConnell DJ
Ref : Molecular & General Genetics , 245 :556 , 1994
Abstract : An extracellular Pseudomonas cepacia lipase, LipA, is inactive when expressed in the absence of the product of the limA gene. Evidence has been presented that LimA is a molecular chaperone. The lipA and limA genes have been cloned in separate and independently inducible expression systems in Escherichia coli. These systems were used to test the molecular chaperone hypothesis by investigating whether LimA could activate presynthesized prelipase and whether presynthesized LimA could activate newly synthesized prelipase. The results show that LimA cannot activate presynthesized prelipase and that presynthesized LimA can activate only a limited number of de novo synthesized prelipase molecules. Co-immunoprecipitation of prelipase/lipase with LimA generated a 1:1 complex of prelipase/lipase and LimA. The results suggest that a 1:1 complex of LipA and LimA is required for prelipase processing and secretion of active lipase.
ESTHER : Aamand_1994_Mol.Gen.Genet_245_556
PubMedSearch : Aamand_1994_Mol.Gen.Genet_245_556
PubMedID: 7528875
Gene_locus related to this paper: burce-lipaa

Related information

Gene_locus related to this paper: burce-lipaa

Citations formats

Aamand JL, Hobson AH, Buckley CM, Jorgensen ST, Diderichsen B, McConnell DJ (1994)
Chaperone-mediated activation in vivo of a Pseudomonas cepacia lipase
Molecular & General Genetics 245 :556

Aamand JL, Hobson AH, Buckley CM, Jorgensen ST, Diderichsen B, McConnell DJ (1994)
Molecular & General Genetics 245 :556