burce-lipaa
Pseudomonas cepacia lipase, Burkholderia sp., lipase (lipA) PCL
Comment
Other strains Pseudomonas cepacia, Burkholderia sp. MC16-3, sp. 99-2-1, strain 383\; Burkholderia cenocepacia || Bornscheuer_1994 Q9R4W9 very short fragment. Another short fragment from Pseudomonas fluorescens is identical Trembl Q9R4V3 Kojima_1994. Burkholderia cepacia (BCL) immobilized on ceramic (Amano PS-C II) or diatomaceous earth (Amano PS-IM)
Relationship
Family : Bacterial_lip_FamI.2
Block : L
Position in NCBI Life Tree : Burkholderia cepacia
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)
> cellular organisms N E > Bacteria N E > Proteobacteria N E > Betaproteobacteria N E > Burkholderiales N E > Burkholderiaceae N E > Burkholderia N E > Burkholderia cepacia complex N E > Burkholderia cepacia N EMolecular evidence
No mutation
1OIL - Pseudomonas cepacia lipase
1YS1 - Burkholderia cepacia lipase complexed with hexylphosphonic acid (R)-2-methyl-3-phenylpropyl ester
1YS2 - Burkholderia cepacia lipase complexed with hexaphosphonic acid (S) 2-methyl-3-phenylpropyl ester
2LIP - Pseudomonas cepacia lipase open conformation
3LIP - Pseudomonas cepacia lipase open conformation
4LIP - Pseudomonas cepacia lipase + Rc-(Rp,Sp)-Dibutylcarbamoylglycero-3-O-Butylphosphonate
5LIP - Pseudomonas cepacia lipase + Rc-(Rp,Sp)-1,2-Dioctylcarbamoylglycero-3-O-Octylphosphonate
No kinetic
No disease
Database
Sequence
Peptide
MARTMRSRVV AGAVACAMSI APFAGTTAVM TLATTHAAMA ATAPAAGYAA TRYPIILVHG LSGTDKYAGV LEYWYGIQED LQQNGATVYV ANLSGFQSDD GPNGRGEQLL AYVKTVLAAT GATKVNLVGH SQGGLSSRYV AAVAPDLVAS VTTIGTPHRG SEFADFVQDV LAYDPTGLSS SVIAAFVNVF GILTSSSHNT NQDALAALQT LTTARAATYN QNYPSAGLGA PGSCQTGAPT ETVGGNTHLL YSWAGTAIQP TLSVFGVTGA TDTSTLPLVD PANVLDLSTL ALFGTGTVMI NRGSGQNDGL VSKCSALYGK VLSTSYKWNH LDEINQLLGV RGAYAEDPVA VIRTHANRLK LAGV
References (28)
| Title : Computational Design of Burkholderia cepacia Lipase Mutants that Show Enhanced Stereoselectivity in the Production of l-Menthol - Ikebe_2025_J.Agric.Food.Chem__ |
| Author(s) : Ikebe J , Yoshida K , Ishihara S , Kurumida Y , Kameda T |
| Ref : Journal of Agricultural and Food Chemistry , : , 2025 |
| Abstract : |
| PubMedSearch : Ikebe_2025_J.Agric.Food.Chem__ |
| PubMedID: 39960458 |
| Gene_locus related to this paper: burce-lipaa |
| Title : Access to valuable building blocks by the regio- and enantioselective ring opening of itaconic anhydride by lipase catalysis - Braa_2022_Org.Biomol.Chem__ |
| Author(s) : Braa N , Merabet-Khelassi M , Toffano M , Guillot R , Aribi-Zouioueche L |
| Ref : Org Biomol Chem , : , 2022 |
| Abstract : |
| PubMedSearch : Braa_2022_Org.Biomol.Chem__ |
| PubMedID: 35293925 |
| Gene_locus related to this paper: burce-lipaa |
| Title : Enhancement of protein thermostability by three consecutive mutations using loop-walking method and machine learning - Yoshida_2021_Sci.Rep_11_11883 |
| Author(s) : Yoshida K , Kawai S , Fujitani M , Koikeda S , Kato R , Ema T |
| Ref : Sci Rep , 11 :11883 , 2021 |
| Abstract : |
| PubMedSearch : Yoshida_2021_Sci.Rep_11_11883 |
| PubMedID: 34088952 |
| Gene_locus related to this paper: burce-lipaa |
| Title : Immobilization of Amano lipase AK from Pseudomonas fluorescens on different types of chitosan-containing supports: use in the kinetic resolution of rac-indanol - de Sousa Fonseca_2021_Bioprocess.Biosyst.Eng__ |
| Author(s) : de Sousa Fonseca T , de Oliveira UMF , de Oliveira M , de Lemos TLG , da Silva MR , Rios NS , Goncalves LRB , de Mattos MC |
| Ref : Bioprocess Biosyst Eng , : , 2021 |
| Abstract : |
| PubMedSearch : de Sousa Fonseca_2021_Bioprocess.Biosyst.Eng__ |
| PubMedID: 33389170 |
| Gene_locus related to this paper: burce-lipaa |
| Title : A facile lipase-catalyzed KR approach toward enantiomerically enriched homopropargyl alcohols - Borowiecki_2019_Bioorg.Chem_93_102754 |
| Author(s) : Borowiecki P , Dranka M |
| Ref : Bioorg Chem , 93 :102754 , 2019 |
| Abstract : |
| PubMedSearch : Borowiecki_2019_Bioorg.Chem_93_102754 |
| PubMedID: 30765117 |
| Gene_locus related to this paper: burce-lipaa |
| Title : Isolation, Cloning and Co-Expression of Lipase and Foldase Genes of Burkholderia territorii GP3 from Mount Papandayan Soil - Putra_2019_J.Microbiol.Biotechnol_29_944 |
| Author(s) : Putra L , Natadiputri G , Meryandini A , Suwanto A |
| Ref : J Microbiol Biotechnol , 29 :944 , 2019 |
| Abstract : |
| PubMedSearch : Putra_2019_J.Microbiol.Biotechnol_29_944 |
| PubMedID: 31091867 |
| Gene_locus related to this paper: burce-lipaa |
| Title : Co-expression, purification and characterization of the lipase and foldase of Burkholderia contaminans LTEB11 - Alnoch_2018_Int.J.Biol.Macromol_116_1222 |
| Author(s) : Alnoch RC , Stefanello AA , Paula Martini V , Richter JL , Mateo C , Souza EM , Mitchell DA , Muller-Santos M , Krieger N |
| Ref : Int J Biol Macromol , 116 :1222 , 2018 |
| Abstract : |
| PubMedSearch : Alnoch_2018_Int.J.Biol.Macromol_116_1222 |
| PubMedID: 29775711 |
| Gene_locus related to this paper: burce-lipaa |
| Title : Lipase-catalyzed kinetic resolution as key step in the synthesis of enantiomerically pure sigma ligands with 2-benzopyran structure - Knappmann_2017_Bioorg.Med.Chem_25_3384 |
| Author(s) : Knappmann I , Lehmkuhl K , Kohler J , Schepmann D , Giera M , Bracher F , Wunsch B |
| Ref : Bioorganic & Medicinal Chemistry , 25 :3384 , 2017 |
| Abstract : |
| PubMedSearch : Knappmann_2017_Bioorg.Med.Chem_25_3384 |
| PubMedID: 28501431 |
| Gene_locus related to this paper: burce-lipaa |
| Title : Cell-bound lipases from Burkholderia sp. ZYB002: gene sequence analysis, expression, enzymatic characterization, and 3D structural model - Shu_2016_BMC.Biotechnol_16_38 |
| Author(s) : Shu Z , Lin H , Shi S , Mu X , Liu Y , Huang J |
| Ref : BMC Biotechnol , 16 :38 , 2016 |
| Abstract : |
| PubMedSearch : Shu_2016_BMC.Biotechnol_16_38 |
| PubMedID: 27142276 |
| Gene_locus related to this paper: burce-lipaa |
| Title : Improving activity and enantioselectivity of lipase via immobilization on macroporous resin for resolution of racemic 1- phenylethanol in non-aqueous medium - Li_2013_BMC.Biotechnol_13_92 |
| Author(s) : Li X , Huang S , Xu L , Yan Y |
| Ref : BMC Biotechnol , 13 :92 , 2013 |
| Abstract : |
| PubMedSearch : Li_2013_BMC.Biotechnol_13_92 |
| PubMedID: 24168516 |
| Gene_locus related to this paper: burce-lipaa |
| Title : Redesign of enzyme for improving catalytic activity and enantioselectivity toward poor substrates: manipulation of the transition state - Ema_2012_Org.Biomol.Chem_10_6299 |
| Author(s) : Ema T , Nakano Y , Yoshida D , Kamata S , Sakai T |
| Ref : Org Biomol Chem , 10 :6299 , 2012 |
| Abstract : |
| PubMedSearch : Ema_2012_Org.Biomol.Chem_10_6299 |
| PubMedID: 22710791 |
| Gene_locus related to this paper: burce-lipaa , burce-q75nt4 |
| Title : Control of lipase enantioselectivity by engineering the substrate binding site and access channel - Lafaquiere_2009_Chembiochem_10_2760 |
| Author(s) : Lafaquiere V , Barbe S , Puech-Guenot S , Guieysse D , Cortes J , Monsan P , Simeon T , Andre I , Remaud-Simeon M |
| Ref : Chembiochem , 10 :2760 , 2009 |
| Abstract : |
| PubMedSearch : Lafaquiere_2009_Chembiochem_10_2760 |
| PubMedID: 19816890 |
| Gene_locus related to this paper: burce-lipaa |
| Title : Purification and properties of the alkaline lipase from Burkholderia cepacia A.T.C.C. 25609 - Dalal_2008_Biotechnol.Appl.Biochem_51_23 |
| Author(s) : Dalal S , Singh PK , Raghava S , Rawat S , Gupta MN |
| Ref : Biotechnol Appl Biochem , 51 :23 , 2008 |
| Abstract : |
| PubMedSearch : Dalal_2008_Biotechnol.Appl.Biochem_51_23 |
| PubMedID: 18052929 |
| Gene_locus related to this paper: burce-lipaa |
| Title : Creation of novel enantioselective lipases by SIMPLEX - Koga_2007_Methods.Mol.Biol_375_165 |
| Author(s) : Koga Y , Yamane T , Nakano H |
| Ref : Methods Mol Biol , 375 :165 , 2007 |
| Abstract : |
| PubMedSearch : Koga_2007_Methods.Mol.Biol_375_165 |
| PubMedID: 17634602 |
| Gene_locus related to this paper: burce-lipaa |
| Title : Role of the N terminus in enzyme activity, stability and specificity in thermophilic esterases belonging to the HSL family - Mandrich_2005_J.Mol.Biol_345_501 |
| Author(s) : Mandrich L , Merone L , Pezzullo M , Cipolla L , Nicotra F , Rossi M , Manco G |
| Ref : Journal of Molecular Biology , 345 :501 , 2005 |
| Abstract : |
| PubMedSearch : Mandrich_2005_J.Mol.Biol_345_501 |
| PubMedID: 15581894 |
| Gene_locus related to this paper: burce-lipaa |
| Title : Mirror-image packing in enantiomer discrimination molecular basis for the enantioselectivity of B.cepacia lipase toward 2-methyl-3-phenyl-1-propanol - Mezzetti_2005_Chem.Biol_12_427 |
| Author(s) : Mezzetti A , Schrag JD , Cheong CS , Kazlauskas RJ |
| Ref : Chemical Biology , 12 :427 , 2005 |
| Abstract : |
| PubMedSearch : Mezzetti_2005_Chem.Biol_12_427 |
| PubMedID: 15850979 |
| Gene_locus related to this paper: burce-lipaa |
| Title : Stereoselective lipases from Burkholderia sp., cloning and their application to preparation of methyl (R)-N-(2,6-dimethylphenyl)alaninate, a key intermediate for (R)-Metalaxyl - Park_2005_J.Biotechnol_120_174 |
| Author(s) : Park OJ , Lee SH |
| Ref : J Biotechnol , 120 :174 , 2005 |
| Abstract : |
| PubMedSearch : Park_2005_J.Biotechnol_120_174 |
| PubMedID: 16102866 |
| Gene_locus related to this paper: 9pseu-a0a0p0s9g4 , burce-lipaa |
| Title : Complex of Burkholderia cepacia lipase with transition state analogue of 1-phenoxy-2-acetoxybutane: biocatalytic, structural and modelling study - Luic_2001_Eur.J.Biochem_268_3964 |
| Author(s) : Luic M , Tomic S , Lescic I , Ljubovic E , Sepac D , Sunjic V , Vitale L , Saenger W , Kojic-Prodic B |
| Ref : European Journal of Biochemistry , 268 :3964 , 2001 |
| Abstract : |
| PubMedSearch : Luic_2001_Eur.J.Biochem_268_3964 |
| PubMedID: 11453990 |
| Gene_locus related to this paper: burce-lipaa |
| Title : Molecular Basis for Enantioselectivity of Lipase from Pseudomonas cepacia toward Primary Alcohols. Modeling, Kinetics, and Chemical Modification of Tyr29 to Increase or Decrease Enantioselectivity - Tuomi_1999_J.Org.Chem_64_2638 |
| Author(s) : Tuomi WV , Kazlauskas RJ |
| Ref : J Org Chem , 64 :2638 , 1999 |
| Abstract : |
| PubMedSearch : Tuomi_1999_J.Org.Chem_64_2638 |
| PubMedID: 11674331 |
| Gene_locus related to this paper: burce-lipaa |
| Title : Structural basis of the chiral selectivity of Pseudomonas cepacia lipase - Lang_1998_Eur.J.Biochem_254_333 |
| Author(s) : Lang DA , Mannesse ML , De Haas GH , Verheij HM , Dijkstra BW |
| Ref : European Journal of Biochemistry , 254 :333 , 1998 |
| Abstract : |
| PubMedSearch : Lang_1998_Eur.J.Biochem_254_333 |
| PubMedID: 9660188 |
| Gene_locus related to this paper: burce-lipaa |
| Title : The open conformation of a Pseudomonas lipase - Schrag_1997_Structure_5_187 |
| Author(s) : Schrag JD , Li Y , Cygler M , Lang D , Burgdorf T , Hecht HJ , Schmid RD , Schomburg D , Rydel TJ , Oliver JD , Strickland LC , Dunaway CM , Larson SB , Day J , McPherson A |
| Ref : Structure , 5 :187 , 1997 |
| Abstract : |
| PubMedSearch : Schrag_1997_Structure_5_187 |
| PubMedID: 9032074 |
| Gene_locus related to this paper: burce-lipaa |
| Title : The crystal structure of a triacylglycerol lipase from Pseudomonas cepacia reveals a highly open conformation in the absence of a bound inhibitor - Kim_1997_Structure_5_173 |
| Author(s) : Kim KK , Song HK , Shin DH , Hwang KY , Suh SW |
| Ref : Structure , 5 :173 , 1997 |
| Abstract : |
| PubMedSearch : Kim_1997_Structure_5_173 |
| PubMedID: 9032073 |
| Gene_locus related to this paper: burce-lipaa |
| Title : Lipase-catalyzed synthesis of arbutin cinnamate in an organic solvent and application of transesterification to stabilize plant pigments - Nakajima_1997_Biosci.Biotechnol.Biochem_61_1926 |
| Author(s) : Nakajima N , Ishihara K , Matsumura S , Hamada H , Nakamura K , Furuya T |
| Ref : Biosci Biotechnol Biochem , 61 :1926 , 1997 |
| Abstract : |
| PubMedSearch : Nakajima_1997_Biosci.Biotechnol.Biochem_61_1926 |
| PubMedID: 9404074 |
| Gene_locus related to this paper: burce-lipaa |
| Title : Lipase of Pseudomonas cepacia for biotechnological purposes: purification, crystallization and characterization - Bornscheuer_1994_Biochim.Biophys.Acta_1201_55 |
| Author(s) : Bornscheuer U , Reif OW , Lausch R , Freitag R , Scheper T , Kolisis FN , Menge U |
| Ref : Biochimica & Biophysica Acta , 1201 :55 , 1994 |
| Abstract : |
| PubMedSearch : Bornscheuer_1994_Biochim.Biophys.Acta_1201_55 |
| PubMedID: 7522571 |
| Gene_locus related to this paper: burce-lipaa |
| Title : Purification and characterization of an alkaline lipase from Pseudomonas fluorescens AK102 - Kojima_1994_Biosci.Biotechnol.Biochem_58_1564 |
| Author(s) : Kojima Y , Yokoe M , Mase T |
| Ref : Biosci Biotechnol Biochem , 58 :1564 , 1994 |
| Abstract : |
| PubMedSearch : Kojima_1994_Biosci.Biotechnol.Biochem_58_1564 |
| PubMedID: 7765474 |
| Gene_locus related to this paper: burce-lipaa |
| Title : Chaperone-mediated activation in vivo of a Pseudomonas cepacia lipase - Aamand_1994_Mol.Gen.Genet_245_556 |
| Author(s) : Aamand JL , Hobson AH , Buckley CM , Jorgensen ST , Diderichsen B , McConnell DJ |
| Ref : Molecular & General Genetics , 245 :556 , 1994 |
| Abstract : |
| PubMedSearch : Aamand_1994_Mol.Gen.Genet_245_556 |
| PubMedID: 7528875 |
| Gene_locus related to this paper: burce-lipaa |
| Title : Activation of a bacterial lipase by its chaperone - Hobson_1993_Proc.Natl.Acad.Sci.U.S.A_90_5682 |
| Author(s) : Hobson AH , Buckley CM , Aamand JL , Jorgensen ST , Diderichsen B , McConnell DJ |
| Ref : Proc Natl Acad Sci U S A , 90 :5682 , 1993 |
| Abstract : |
| PubMedSearch : Hobson_1993_Proc.Natl.Acad.Sci.U.S.A_90_5682 |
| PubMedID: 7685908 |
| Gene_locus related to this paper: burce-lipaa |
| Title : Cloning, sequence, and expression of a lipase gene from Pseudomonas cepacia: lipase production in heterologous hosts requires two Pseudomonas genes - Jorgensen_1991_J.Bacteriol_173_559 |
| Author(s) : Jorgensen S , Skov KW , Diderichsen B |
| Ref : Journal of Bacteriology , 173 :559 , 1991 |
| Abstract : |
| PubMedSearch : Jorgensen_1991_J.Bacteriol_173_559 |
| PubMedID: 1987151 |
| Gene_locus related to this paper: burce-lipaa |