Alhomida_2000_Toxicology_147_33

Reference

Title : Kinetic analysis of the toxicological effect of tacrine (Cognex) on human retinal acetylcholinesterase activity - Alhomida_2000_Toxicology_147_33
Author(s) : Alhomida AS , Al-Rajhi AA , Kamal MA , Al-Jafari AA
Ref : Toxicology , 147 :33 , 2000
Abstract : For the first time, kinetic parameters of the effect of tacrine, an anti-cholinesterase inhibitor of therapeutic potential in Alzheimer's disease has been studied on human retinal acetyl-cholinesterase (AChE). Tacrine inhibited the AChE activity in a concentration dependent manner, the IC(50) being about 45 nM. The Michaelis-Menten constant (K(m)) for the hydrolysis of acetylthiocholine iodide was found to be 0.120 mM and this value was increased by 4-52.8% in the presence of tacrine. V(max) was observed to be 2.23 micromol/h per mg protein for the control system, while it was decreased by 14.73-56.25% in the tacrine treated systems. Dixon as well as Lineweaver-Burk plots and their secondary replots indicated that the nature of the inhibition was of the mixed type, i. e. a combination of competitive and noncompetitive inhibition. The values of K(i) and K(I) were estimated to be as 37.76 and 64.36 nM, respectively.
ESTHER : Alhomida_2000_Toxicology_147_33
PubMedSearch : Alhomida_2000_Toxicology_147_33
PubMedID: 10837930

Related information

Citations formats

Alhomida AS, Al-Rajhi AA, Kamal MA, Al-Jafari AA (2000)
Kinetic analysis of the toxicological effect of tacrine (Cognex) on human retinal acetylcholinesterase activity
Toxicology 147 :33

Alhomida AS, Al-Rajhi AA, Kamal MA, Al-Jafari AA (2000)
Toxicology 147 :33