| Title : Regioselective deacetylation of cellulose acetates by acetyl xylan esterases of different CE-families - Altaner_2003_J.Biotechnol_105_95 |
| Author(s) : Altaner C , Saake B , Tenkanen M , Eyzaguirre J , Faulds CB , Biely P , Viikari L , Siika-aho M , Puls J |
| Ref : J Biotechnol , 105 :95 , 2003 |
| Abstract : Cellulose acetate (CA) was found to be a substrate of several acetyl xylan esterases (AXE). Eight AXE from different carbohydrate esterase (CE) families were tested on their activity against CA with a degree of substitution of 0.7 and 1.4. The classification of the AXEs into CE families according to their structure by hydrophobic cluster analysis followed clearly their activity against CA. Within the same CE family similar, and between the CE families different deacetylation behaviours could be observed. Furthermore, each esterase family showed a distinct regioselective mode of action. The CE 1 family enzymes regioselectively cleaved the substituents in C2- and C3-position, while CE 5 family enzymes only cleaved the acetyl groups in C2-position. CE 4 family enzymes seemed to interact only with the substituents in C3-position. Evidence was found that the deacetylation reaction of the CE 1 family enzymes proceeded faster in C2- than in C3-position of CA. The enzymes were able to cleave acetyl groups from fully substituted anhydroglucose units. |
| ESTHER : Altaner_2003_J.Biotechnol_105_95 |
| PubMedSearch : Altaner_2003_J.Biotechnol_105_95 |
| PubMedID: 14511913 |
Altaner C, Saake B, Tenkanen M, Eyzaguirre J, Faulds CB, Biely P, Viikari L, Siika-aho M, Puls J (2003)
Regioselective deacetylation of cellulose acetates by acetyl xylan esterases of different CE-families
J Biotechnol
105 :95
Altaner C, Saake B, Tenkanen M, Eyzaguirre J, Faulds CB, Biely P, Viikari L, Siika-aho M, Puls J (2003)
J Biotechnol
105 :95