| Title : Site-specific analysis of protein hydration based on unnatural amino acid fluorescence - Amaro_2015_J.Am.Chem.Soc_137_4988 |
| Author(s) : Amaro M , Brezovsky J , Kovacova S , Sykora J , Bednar D , Nemec V , Liskova V , Kurumbang NP , Beerens K , Chaloupkova R , Paruch K , Hof M , Damborsky J |
| Ref : Journal of the American Chemical Society , 137 :4988 , 2015 |
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Abstract :
Hydration of proteins profoundly affects their functions. We describe a simple and general method for site-specific analysis of protein hydration based on the in vivo incorporation of fluorescent unnatural amino acids and their analysis by steady-state fluorescence spectroscopy. Using this method, we investigate the hydration of functionally important regions of dehalogenases. The experimental results are compared to findings from molecular dynamics simulations. |
| PubMedSearch : Amaro_2015_J.Am.Chem.Soc_137_4988 |
| PubMedID: 25815779 |
Amaro M, Brezovsky J, Kovacova S, Sykora J, Bednar D, Nemec V, Liskova V, Kurumbang NP, Beerens K, Chaloupkova R, Paruch K, Hof M, Damborsky J (2015)
Site-specific analysis of protein hydration based on unnatural amino acid fluorescence
Journal of the American Chemical Society
137 :4988
Amaro M, Brezovsky J, Kovacova S, Sykora J, Bednar D, Nemec V, Liskova V, Kurumbang NP, Beerens K, Chaloupkova R, Paruch K, Hof M, Damborsky J (2015)
Journal of the American Chemical Society
137 :4988