Avilan_2023_ChemSusChem__e202202277

Reference

Title : Concentration-dependent inhibition of mesophilic PETases on poly(ethylene terephthalate) can be eliminated by enzyme engineering - Avilan_2023_ChemSusChem__e202202277
Author(s) : Avilan L , Lichtenstein BR , Koenig G , Zahn M , Allen MD , Oliveira L , Clark M , Bemmer V , Graham R , Austin HP , Dominick G , Johnson CW , Beckham GT , McGeehan J , Pickford AR
Ref : ChemSusChem , :e202202277 , 2023
Abstract :

Enzyme-based depolymerization is a viable approach for recycling of poly(ethylene terephthalate) (PET). PETase from Ideonella sakaiensis (IsPETase) is capable of PET hydrolysis under mild conditions but suffers from concentration-dependent inhibition. Here, we report that this inhibition is dependent on incubation time, the solution conditions and PET surface area. Furthermore, this inhibition is evident in other mesophilic PET-degrading enzymes to varying degrees, independent of the level of PET depolymerization activity. The inhibition has no clear structural basis, but moderately thermostable IsPETase variants exhibit reduced inhibition, and the property is completely absent in the highly thermostable HotPETase, previously engineered by directed evolution, which our simulations suggest results from reduced flexibility around the active site. This work highlights a limitation in applying natural mesophilic hydrolases for PET hydrolysis, and reveals an unexpected positive outcome of engineering these enzymes for enhanced thermostability.

PubMedSearch : Avilan_2023_ChemSusChem__e202202277
PubMedID: 36811288
Gene_locus related to this paper: psea4-u2z2l5 , 9gamm-a0a031mkr8 , 9burk-a0a1w6l438 , 9psed-a0a078mgg8 , pseol-e9kjl1 , 9burk-a0a0g3bi90 , 9burk-a0a1w6l588 , idesa-peth , acide-PBSA

Citations formats

Avilan L, Lichtenstein BR, Koenig G, Zahn M, Allen MD, Oliveira L, Clark M, Bemmer V, Graham R, Austin HP, Dominick G, Johnson CW, Beckham GT, McGeehan J, Pickford AR (2023)
Concentration-dependent inhibition of mesophilic PETases on poly(ethylene terephthalate) can be eliminated by enzyme engineering
ChemSusChem :e202202277

Avilan L, Lichtenstein BR, Koenig G, Zahn M, Allen MD, Oliveira L, Clark M, Bemmer V, Graham R, Austin HP, Dominick G, Johnson CW, Beckham GT, McGeehan J, Pickford AR (2023)
ChemSusChem :e202202277