Bergeret_2012_J.Biol.Chem_287_33675

Reference

Title : Biochemical and structural study of the atypical acyltransferase domain from the mycobacterial polyketide synthase Pks13 - Bergeret_2012_J.Biol.Chem_287_33675
Author(s) : Bergeret F , Gavalda S , Chalut C , Malaga W , Quemard A , Pedelacq JD , Daffe M , Guilhot C , Mourey L , Bon C
Ref : Journal of Biological Chemistry , 287 :33675 , 2012
Abstract :

Pks13 is a type I polyketide synthase involved in the final biosynthesis step of mycolic acids, virulence factors, and essential components of the Mycobacterium tuberculosis envelope. Here, we report the biochemical and structural characterization of a 52-kDa fragment containing the acyltransferase domain of Pks13. This fragment retains the ability to load atypical extender units, unusually long chain acyl-CoA with a predilection for carboxylated substrates. High resolution crystal structures were determined for the apo, palmitoylated, and carboxypalmitoylated forms. Structural conservation with type I polyketide synthases and related fatty-acid synthases also extends to the interdomain connections. Subtle changes could be identified both in the active site and in the upstream and downstream linkers in line with the organization displayed by this singular polyketide synthase. More importantly, the crystallographic analysis illustrated for the first time how a long saturated chain can fit in the core structure of an acyltransferase domain through a dedicated channel. The structures also revealed the unexpected binding of a 12-mer peptide that might provide insight into domain-domain interaction.

PubMedSearch : Bergeret_2012_J.Biol.Chem_287_33675
PubMedID: 22825853
Gene_locus related to this paper: myctu-PKS13

Related information

Gene_locus myctu-PKS13

Citations formats

Bergeret F, Gavalda S, Chalut C, Malaga W, Quemard A, Pedelacq JD, Daffe M, Guilhot C, Mourey L, Bon C (2012)
Biochemical and structural study of the atypical acyltransferase domain from the mycobacterial polyketide synthase Pks13
Journal of Biological Chemistry 287 :33675

Bergeret F, Gavalda S, Chalut C, Malaga W, Quemard A, Pedelacq JD, Daffe M, Guilhot C, Mourey L, Bon C (2012)
Journal of Biological Chemistry 287 :33675