Bessereau_1998_J.Biol.Chem_273_12786

Reference

Title : Nonmyogenic factors bind nicotinic acetylcholine receptor promoter elements required for response to denervation - Bessereau_1998_J.Biol.Chem_273_12786
Author(s) : Bessereau JL , Laudenbach V , Le Poupon C , Changeux JP
Ref : Journal of Biological Chemistry , 273 :12786 , 1998
Abstract :

Nicotinic acetylcholine receptors (AChRs) belong to a class of muscle proteins whose expression is regulated by muscle electrical activity. In innervated muscle fiber, AChR genes are transcriptionally repressed outside of the synapse, while after denervation they become reexpressed throughout the fiber. The myogenic determination factors (MDFs) of the MyoD family have been shown to play a central role in this innervation-dependent regulation. In the chicken AChR alpha-subunit gene promoter, two E-boxes that bind MDFs are necessary to achieve the enhancement of transcription following muscle denervation. However, the deletion of promoter sequences located upstream to these E-boxes greatly impairs the response to denervation (Bessereau, J. L., Stratford- Perricaudet, L. D., Piette, J., Le Poupon, C. and Changeux, J. P. (1994) Proc. Natl. Acad. Sci. U. S. A. 91, 1304-1308). Here we identified two additional cis-regulatory elements of the alpha-subunit gene promoter that cooperate with the E-boxes in the denervation response. One region binds the Sp1 and Sp3 zinc finger transcription factors. The second region binds at least three distinct factors, among which we identified an upstream stimulatory factor, a b-ZIP-HLH transcription factor. We propose that among MDF-responsive muscle promoters, a specific combination between myogenic and nonmyogenic factors specify innervation-dependent versus innervation-independent promoters.

PubMedSearch : Bessereau_1998_J.Biol.Chem_273_12786
PubMedID: 9582305

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Citations formats

Bessereau JL, Laudenbach V, Le Poupon C, Changeux JP (1998)
Nonmyogenic factors bind nicotinic acetylcholine receptor promoter elements required for response to denervation
Journal of Biological Chemistry 273 :12786

Bessereau JL, Laudenbach V, Le Poupon C, Changeux JP (1998)
Journal of Biological Chemistry 273 :12786