| Title : Cloning, expression, purification, crystallization and preliminary X-ray studies of epoxide hydrolases A and B from Mycobacterium tuberculosis - Biswal_2006_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_62_136 |
| Author(s) : Biswal BK , Garen G , Cherney MM , Garen C , James MN |
| Ref : Acta Crystallographica Sect F Struct Biol Cryst Commun , 62 :136 , 2006 |
|
Abstract :
Mycobacterium tuberculosis epoxide hydrolases A and B, corresponding to open reading frames Rv3617 and Rv1938, are detoxification enzymes against epoxides. The recombinant forms of these enzymes have been expressed in Escherichia coli and purified to homogeneity. Diffraction-quality crystals of Rv3617 and Rv1938 were obtained by the hanging-drop vapour-diffusion technique. Crystals of Rv3617 and Rv1938 diffracted to 3.0 and 2.1 A resolution, respectively, at the ALS synchrotron at Berkeley, CA, USA. |
| PubMedSearch : Biswal_2006_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_62_136 |
| PubMedID: 16511284 |
| Gene_locus related to this paper: myctu-ephB |
| Inhibitor | Diphenylurea |
| Gene_locus | myctu-ephB |
| Family | Epoxide_hydrolase |
| Structure | 2E3J 2ZJF |
Biswal BK, Garen G, Cherney MM, Garen C, James MN (2006)
Cloning, expression, purification, crystallization and preliminary X-ray studies of epoxide hydrolases A and B from Mycobacterium tuberculosis
Acta Crystallographica Sect F Struct Biol Cryst Commun
62 :136
Biswal BK, Garen G, Cherney MM, Garen C, James MN (2006)
Acta Crystallographica Sect F Struct Biol Cryst Commun
62 :136