Boheim_1981_Proc.Natl.Acad.Sci.U.S.A_78_3586

Reference

Title : Agonist-activated ionic channels in acetylcholine receptor reconstituted into planar lipid bilayers - Boheim_1981_Proc.Natl.Acad.Sci.U.S.A_78_3586
Author(s) : Boheim G , Hanke W , Barrantes FJ , Eibl H , Sakmann B , Fels G , Maelicke A
Ref : Proc Natl Acad Sci U S A , 78 :3586 , 1981
Abstract :

Planar lipid bilayers were formed with the mixed chain phospholipid 1-stearoyl-3-myristolglycero-2-phosphocholine. Acetylcholine receptor membrane fragments or the purified receptor protein was incorporated into these bilayers by fusing receptor-containing vesicles with the planar membranes a few degrees below the lipid phase transition temperature. Single-channel currents activated by nicotinic agonists in the reconstituted system resembled those observed in intact rat and frog muscle membrane as measured by the patch clamp technique. The observed channel characteristics did not depend on the degree of receptor purification. Thus, the receptor-enriched fragments and those depleted of nonreceptor peripheral peptides, the purified receptor monomer/dimer mixtures, and the isolated receptor monomer as defined by gel electrophoresis all shared similar electrochemical properties in the synthetic lipid bilayer. The agonist-activated ionic channel seems, therefore, to be contained within the receptor monomer.

PubMedSearch : Boheim_1981_Proc.Natl.Acad.Sci.U.S.A_78_3586
PubMedID: 6267599

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Citations formats

Boheim G, Hanke W, Barrantes FJ, Eibl H, Sakmann B, Fels G, Maelicke A (1981)
Agonist-activated ionic channels in acetylcholine receptor reconstituted into planar lipid bilayers
Proc Natl Acad Sci U S A 78 :3586

Boheim G, Hanke W, Barrantes FJ, Eibl H, Sakmann B, Fels G, Maelicke A (1981)
Proc Natl Acad Sci U S A 78 :3586