| Title : Study of G-protein-coupled receptor-protein interactions using gel overlay assays and glutathione-S-transferase-fusion protein pull-downs - Brady_2004_Methods.Mol.Biol_259_371 |
| Author(s) : Brady AE , Wang Q , Limbird LE |
| Ref : Methods Mol Biol , 259 :371 , 2004 |
|
Abstract :
Numerous recent studies have suggested that the predicted cytosolic domains of G-protein-coupled receptors (GPCRs) represent a surface for association with proteins that may serve multiple roles in receptor localization, turnover, and signaling beyond the well-characterized interactions of these receptors with heterotrimeric G-proteins. This chapter describes two in vitro methods for ascertaining interactions between GPCRs and various binding partners: gel overlay strategies and GST-fusion protein pull-downs. |
| PubMedSearch : Brady_2004_Methods.Mol.Biol_259_371 |
| PubMedID: 15250505 |
Brady AE, Wang Q, Limbird LE (2004)
Study of G-protein-coupled receptor-protein interactions using gel overlay assays and glutathione-S-transferase-fusion protein pull-downs
Methods Mol Biol
259 :371
Brady AE, Wang Q, Limbird LE (2004)
Methods Mol Biol
259 :371