| Title : Study of GPCR-protein interactions using gel overlay assays and glutathione-S-transferase-fusion protein pull-downs - Brady_2011_Methods.Mol.Biol_746_347 |
| Author(s) : Brady AE , Chen Y , Limbird LE , Wang Q |
| Ref : Methods Mol Biol , 746 :347 , 2011 |
|
Abstract :
Numerous recent studies have suggested that the predicted cytosolic domains of G protein-coupled receptors represent a surface for association with proteins that may serve multiple roles in receptor localization, turnover, and signaling beyond the well-characterized interactions of these receptors with heterotrimeric G proteins. This Chapter describes two in vitro methods for ascertaining interactions between G protein-coupled receptors and various binding partners: gel overlay strategies and GST-fusion protein pull-downs. |
| PubMedSearch : Brady_2011_Methods.Mol.Biol_746_347 |
| PubMedID: 21607867 |
Brady AE, Chen Y, Limbird LE, Wang Q (2011)
Study of GPCR-protein interactions using gel overlay assays and glutathione-S-transferase-fusion protein pull-downs
Methods Mol Biol
746 :347
Brady AE, Chen Y, Limbird LE, Wang Q (2011)
Methods Mol Biol
746 :347