Butcher_2011_J.Biol.Chem_286_11506

Reference

Title : Differential G-protein-coupled receptor phosphorylation provides evidence for a signaling bar code - Butcher_2011_J.Biol.Chem_286_11506
Author(s) : Butcher AJ , Prihandoko R , Kong KC , McWilliams P , Edwards JM , Bottrill A , Mistry S , Tobin AB
Ref : Journal of Biological Chemistry , 286 :11506 , 2011
Abstract :

G-protein-coupled receptors are hyper-phosphorylated in a process that controls receptor coupling to downstream signaling pathways. The pattern of receptor phosphorylation has been proposed to generate a "bar code" that can be varied in a tissue-specific manner to direct physiologically relevant receptor signaling. If such a mechanism existed, receptors would be expected to be phosphorylated in a cell/tissue-specific manner. Using tryptic phosphopeptide maps, mass spectrometry, and phospho-specific antibodies, it was determined here that the prototypical G(q/11)-coupled M(3)-muscarinic receptor was indeed differentially phosphorylated in various cell and tissue types supporting a role for differential receptor phosphorylation in directing tissue-specific signaling. Furthermore, the phosphorylation profile of the M(3)-muscarinic receptor was also dependent on the stimulus. Full and partial agonists to the M(3)-muscarinic receptor were observed to direct phosphorylation preferentially to specific sites. This hitherto unappreciated property of ligands raises the possibility that one mechanism underlying ligand bias/functional selectivity, a process where ligands direct receptors to preferred signaling pathways, may be centered on the capacity of ligands to promote receptor phosphorylation at specific sites.

PubMedSearch : Butcher_2011_J.Biol.Chem_286_11506
PubMedID: 21177246

Related information

Citations formats

Butcher AJ, Prihandoko R, Kong KC, McWilliams P, Edwards JM, Bottrill A, Mistry S, Tobin AB (2011)
Differential G-protein-coupled receptor phosphorylation provides evidence for a signaling bar code
Journal of Biological Chemistry 286 :11506

Butcher AJ, Prihandoko R, Kong KC, McWilliams P, Edwards JM, Bottrill A, Mistry S, Tobin AB (2011)
Journal of Biological Chemistry 286 :11506