Chao_2016_Chem.Commun.(Camb)_52_11599

Reference

Title : A selective and sensitive near-infrared fluorescent probe for acetylcholinesterase imaging - Chao_2016_Chem.Commun.(Camb)_52_11599
Author(s) : Chao S , Krejci E , Bernard V , Leroy J , Jean L , Renard PY
Ref : Chem Commun (Camb) , 52 :11599 , 2016
Abstract : Two near infra-red (NIR) fluorescent probes HupNIR1 and HupNIR2 based on the huprine scaffold and cyanine 5.0 dye have been synthesised and evaluated in situ for the detection of acetylcholinesterases in different tissues. As anticipated by the initial properties of huprine, both probes displayed a high affinity and selectivity for AChE toward BChE, with IC50 values in the nanomolar range and without any non-specific binding in the tissues. HupNIR2 appears the best probe for AChE with a great selectivity and sensitivity for AChE even in the brain region displaying a low AChE concentration as striatum. Moreover, the binding of HupNIR2 is affected when AChE is inhibited with toxic molecules such as organophosphates. This work provides a new tool to visualize active AChE in biological applications.
ESTHER : Chao_2016_Chem.Commun.(Camb)_52_11599
PubMedSearch : Chao_2016_Chem.Commun.(Camb)_52_11599
PubMedID: 27604478

Related information

Citations formats

Chao S, Krejci E, Bernard V, Leroy J, Jean L, Renard PY (2016)
A selective and sensitive near-infrared fluorescent probe for acetylcholinesterase imaging
Chem Commun (Camb) 52 :11599

Chao S, Krejci E, Bernard V, Leroy J, Jean L, Renard PY (2016)
Chem Commun (Camb) 52 :11599

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    [paper] => Chao_2016_Chem.Commun.(Camb)_52_11599
    [author] => Chao S || Krejci E || Bernard V || Leroy J || Jean L || Renard PY
    [year] => 2016
    [title] => A selective and sensitive near-infrared fluorescent probe for acetylcholinesterase imaging
    [journal] => Chem Commun (Camb)
    [volume] => 52
    [page] => 11599
    [medline] => 27604478
    [abstract] => Chao_2016_Chem.Commun.(Camb)_52_11599
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            [content] => Two near infra-red (NIR) fluorescent probes HupNIR1 and HupNIR2 based on the huprine scaffold and cyanine 5.0 dye have been synthesised and evaluated in situ for the detection of acetylcholinesterases in different tissues. As anticipated by the initial properties of huprine, both probes displayed a high affinity and selectivity for AChE toward BChE, with IC50 values in the nanomolar range and without any non-specific binding in the tissues. HupNIR2 appears the best probe for AChE with a great selectivity and sensitivity for AChE even in the brain region displaying a low AChE concentration as striatum. Moreover, the binding of HupNIR2 is affected when AChE is inhibited with toxic molecules such as organophosphates. This work provides a new tool to visualize active AChE in biological applications.
        )

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