Charbonneau_2010_J.Biochem_148_299

Reference

Title : A novel thermostable carboxylesterase from Geobacillus thermodenitrificans: evidence for a new carboxylesterase family - Charbonneau_2010_J.Biochem_148_299
Author(s) : Charbonneau DM , Meddeb-Mouelhi F , Beauregard M
Ref : J Biochem , 148 :299 , 2010
Abstract :

A novel gene encoding an esterase from Geobacillus thermodenitrificans strain CMB-A2 was cloned, sequenced and functionally expressed in Escherichia coli M15. Sequence analysis revealed an open reading frame of 747 bp corresponding to a polypeptide of 249 amino acid residues (named EstGtA2). After purification, a specific activity of 2.58 U mg(-1) was detected using p-NP caprylate (C8) at 50 degrees C and pH 8.0 (optimal conditions). The enzyme catalyses the hydrolysis of triglycerides (tributyrin) and a variety of p-nitrophenyl esters with different fatty acyl chain length (C4-C16). The enzyme has potential for various industrial applications since it is characterized by its activity under a wide range of pH, from 25 to 65 degrees C. Using Geobacillus stearothermophilus Est30 esterase structure as template, a model of EstGtA2 was built using ESyPred3D. Analysis of this structural model allowed identifying putative sequence features that control EstGtA2 enzymatic properties. Based on sequence properties, multiple sequence comparisons and phylogenetic analyses, this enzyme appears to belong to a new family of carboxylesterases.

PubMedSearch : Charbonneau_2010_J.Biochem_148_299
PubMedID: 20587647
Gene_locus related to this paper: geotd-g3jwz2

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Citations formats

Charbonneau DM, Meddeb-Mouelhi F, Beauregard M (2010)
A novel thermostable carboxylesterase from Geobacillus thermodenitrificans: evidence for a new carboxylesterase family
J Biochem 148 :299

Charbonneau DM, Meddeb-Mouelhi F, Beauregard M (2010)
J Biochem 148 :299