| Title : Efficient biodegradation of highly crystallized polyethylene terephthalate through cell surface display of bacterial PETase - Chen_2020_Sci.Total.Environ_709_136138 |
| Author(s) : Chen Z , Wang Y , Cheng Y , Wang X , Tong S , Yang H , Wang Z |
| Ref : Sci Total Environ , 709 :136138 , 2020 |
| Abstract : Polyethylene terephthalate (PET) is one of the most widely used plastics in the world. Accumulation of the discarded PET in the environment is creating a global environmental problem. Recently, a bacterial enzyme named PETase was found to have the novel ability to degrade the highly crystallized PET. However, the enzymatic activity of native PETase is still low limiting its possible use in recycling of PET. In this study, we developed a whole-cell biocatalyst by displaying PETase on the surface of yeast (Pichia pastoris) cell to improve its degradation efficiency. Our data shows that PETase could be functionally displayed on the yeast cell with enhanced pH and thermal stability. The turnover rate of the PETase-displaying yeast whole-cell biocatalyst towards highly crystallized PET dramatically increased about 36-fold compared with that of purified PETase. Furthermore, the whole-cell biocatalyst showed stable turnover rate after seven repeated use and under some chemical/solvent conditions, and its ability to degrade different commercial highly crystallized PET bottles. Our results reveal that PETase-displaying whole-cell biocatalyst affords a promising route for efficient biological recycling of PET. |
| ESTHER : Chen_2020_Sci.Total.Environ_709_136138 |
| PubMedSearch : Chen_2020_Sci.Total.Environ_709_136138 |
| PubMedID: 31887523 |
Chen Z, Wang Y, Cheng Y, Wang X, Tong S, Yang H, Wang Z (2020)
Efficient biodegradation of highly crystallized polyethylene terephthalate through cell surface display of bacterial PETase
Sci Total Environ
709 :136138
Chen Z, Wang Y, Cheng Y, Wang X, Tong S, Yang H, Wang Z (2020)
Sci Total Environ
709 :136138