Title : Curcumin analog C16 attenuates bone cancer pain induced by MADB 106 breast cancer cells in female rats and inhibits the CREB\/NLGN2 signaling axis by targeting CaMKalpha - Chen_2024_Neuropharmacol_266_110284 |
Author(s) : Chen L , Ni C , Lu D , Zhang S , Li Y , Wang D , Hua B , Ni H , Xu L , Yao M |
Ref : Neuropharmacology , 266 :110284 , 2024 |
Abstract :
Bone cancer pain (BCP) is one of the most severe complications faced by patients with cancer; however, current pharmacological options are limited. Curcumin has been demonstrated to possess anti-inflammatory and analgesic properties; however, our preliminary research found that the analgesic efficiency of curcumin is not high in BCP. Consequently, curcumin analogs have emerged as a significant focus of our research. This study aimed to systematically investigate the analgesic effects of C16 in rats with BCP induced by MADB 106 breast cancer cells (MADB 106-induced BCP) and elucidate the underlying molecular mechanisms. A range of experimental methods, including kinase profiling, transcriptome sequencing, behavioral tests, immunofluorescence, and biochemical analyses, were employed to comprehensively assess the role of C16 in the MADB 106-induced BCP model. The results indicated that C16 significantly alleviated bone cancer pain induced by Luciferin-MADB 106 cells (10^6 cells) in a dose-dependent manner. Importantly, kinase profiling and validation experiments identified CaMKIalpha in spinal dorsal horn neurons as the primary target of C16's analgesic effect on MADB 106-induced BCP. Continuous intrathecal administration of C16 markedly suppressed the expression of CREB and P-CREB and reduced the expression of neuroligin 2 in the spinal cords of BCP rats, thereby clarifying the mechanism of action of C16 in alleviating MADB 106-induced BCP. These findings suggest that C16 possesses significant therapeutic potential for mitigating MADB 106-induced BCP nociceptive hypersensitivity, providing a foundation for the future development of novel drugs targeting MADB 106-induced BCP. |
PubMedSearch : Chen_2024_Neuropharmacol_266_110284 |
PubMedID: 39725125 |
Chen L, Ni C, Lu D, Zhang S, Li Y, Wang D, Hua B, Ni H, Xu L, Yao M (2024)
Curcumin analog C16 attenuates bone cancer pain induced by MADB 106 breast cancer cells in female rats and inhibits the CREB\/NLGN2 signaling axis by targeting CaMKalpha
Neuropharmacology
266 :110284
Chen L, Ni C, Lu D, Zhang S, Li Y, Wang D, Hua B, Ni H, Xu L, Yao M (2024)
Neuropharmacology
266 :110284