| Title : Mass changes of inositol(1,4,5)trisphosphate in trachealis muscle following agonist stimulation - Chilvers_1989_Eur.J.Pharmacol_164_587 |
| Author(s) : Chilvers ER , Challiss RA , Barnes PJ , Nahorski SR |
| Ref : European Journal of Pharmacology , 164 :587 , 1989 |
|
Abstract :
Agonist-stimulated changes in D-Ins(1,4,5)P3 has been examined in tracheal smooth muscle using a specific radioreceptor assay utilizing the binding of [3H] or [32P]Ins(1,4,5)P3 to a bovine adrenal cortex preparation. Carbachol produced a maximal increase in Ins(1,4,5)P3 concentration after 5 s (basal: 12.9 +/- 0.8; stimulated: 27 +/- 1.5 pmol/mg protein) with an EC50 of 6.7 +/- 0.8 microM. With longer stimulation periods Ins(1,4,5)P3 rapidly returned to basal values by 30 s. This is the first report of mass measurements of Ins(1,4,5)P3 in smooth muscle. |
| PubMedSearch : Chilvers_1989_Eur.J.Pharmacol_164_587 |
| PubMedID: 2548874 |
Chilvers ER, Challiss RA, Barnes PJ, Nahorski SR (1989)
Mass changes of inositol(1,4,5)trisphosphate in trachealis muscle following agonist stimulation
European Journal of Pharmacology
164 :587
Chilvers ER, Challiss RA, Barnes PJ, Nahorski SR (1989)
European Journal of Pharmacology
164 :587