Colovic_2010_Toxicol.Lett_193_9

Reference

Title : Toxic effects of diazinon and its photodegradation products - Colovic_2010_Toxicol.Lett_193_9
Author(s) : Colovic M , Krstic D , Petrovic S , Leskovac A , Joksic G , Savic J , Franko M , Trebse P , Vasic V
Ref : Toxicol Lett , 193 :9 , 2010
Abstract :

The toxic effects of diazinon and its irradiated solutions were investigated using cultivated human blood cells (lymphocytes and erythrocytes) and skin fibroblasts. Ultra Performance Liquid Chromatography (UPLC)-UV/VIS system was used to monitor the disappearance of starting diazinon during 115-min photodegradation and formation of its by-products (diazoxon and 2-isopropyl-6-methyl-4-pyrimidinol (IMP)) as a function of time. Dose-dependent AChE and Na(+)/K(+)-ATPase inhibition by diazinon was obtained for all investigated cells. Calculated IC(50) (72 h) values, in M, were: 7.5x10(-6)/3.4x10(-5), 8.7x10(-5)/6.6x10(-5), and 3.0x10(-5)/4.6x10(-5) for fibroblast, erythrocyte and lymphocyte AChE/Na(+)/K(+)-ATPase, respectively. Results obtained for reference commercially purified target enzymes indicate similar sensitivity of AChE towards diazinon (IC(50) (20 min)-7.8x10(-5)M), while diazinon concentrations below 10mM did not noticeably affect Na(+)/K(+)-ATPase activity. Besides, diazinon and IMP induced increasing incidence of micronuclei (via clastogenic mode of action) in a dose-dependent manner up to 2x10(-6)M and significant inhibition of cell proliferation and increased level of malondialdehyde at all investigated concentrations. Although after 15-min diazinon irradiation formed products do not affect purified commercial enzymes activities, inhibitory effect of irradiated solutions on cell enzymes increased as a function of time exposure to UV light and resulted in significant reduction of AChE (up to 28-45%) and Na(+)/K(+)-ATPase (up to 35-40%) at the end of irradiation period. Moreover, photodegradation treatment strengthened prooxidative properties of diazinon as well as its potency to induce cytogenetic damage.

PubMedSearch : Colovic_2010_Toxicol.Lett_193_9
PubMedID: 19948211

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Citations formats

Colovic M, Krstic D, Petrovic S, Leskovac A, Joksic G, Savic J, Franko M, Trebse P, Vasic V (2010)
Toxic effects of diazinon and its photodegradation products
Toxicol Lett 193 :9

Colovic M, Krstic D, Petrovic S, Leskovac A, Joksic G, Savic J, Franko M, Trebse P, Vasic V (2010)
Toxicol Lett 193 :9