| Title : A simplified procedure for the purification of large quantities of fetal bovine serum acetylcholinesterase - De La Hoz_1986_Life.Sci_39_195 |
| Author(s) : De La Hoz DM , Doctor BP , Ralston JS , Rush RS , Wolfe AD |
| Ref : Life Sciences , 39 :195 , 1986 |
|
Abstract :
A simple procedure has been developed for the large scale purification of fetal bovine serum acetylcholinesterase (AChE) (EC 3.1.1.7). The procedure involves two steps: batch adsorption of the AChE from 250 L of serum onto a procainamide affinity Sepharose 4B gel; and analytical procainamide affinity chromatography of the step-1 product. Over 100 mg of AChE was purified in 10 days to apparent homogeneity with this procedure. |
| PubMedSearch : De La Hoz_1986_Life.Sci_39_195 |
| PubMedID: 3736320 |
| Inhibitor | Procainamide |
De La Hoz DM, Doctor BP, Ralston JS, Rush RS, Wolfe AD (1986)
A simplified procedure for the purification of large quantities of fetal bovine serum acetylcholinesterase
Life Sciences
39 :195
De La Hoz DM, Doctor BP, Ralston JS, Rush RS, Wolfe AD (1986)
Life Sciences
39 :195