Deo_2021_Nat.Chem.Biol_17_718

Reference

Title : The HaloTag as a general scaffold for far-red tunable chemigenetic indicators - Deo_2021_Nat.Chem.Biol_17_718
Author(s) : Deo C , Abdelfattah AS , Bhargava HK , Berro AJ , Falco N , Farrants H , Moeyaert B , Chupanova M , Lavis LD , Schreiter ER
Ref : Nat Chemical Biology , 17 :718 , 2021
Abstract :

Functional imaging using fluorescent indicators has revolutionized biology, but additional sensor scaffolds are needed to access properties such as bright, far-red emission. Here, we introduce a new platform for 'chemigenetic' fluorescent indicators, utilizing the self-labeling HaloTag protein conjugated to environmentally sensitive synthetic fluorophores. We solve a crystal structure of HaloTag bound to a rhodamine dye ligand to guide engineering efforts to modulate the dye environment. We show that fusion of HaloTag with protein sensor domains that undergo conformational changes near the bound dye results in large and rapid changes in fluorescence output. This generalizable approach affords bright, far-red calcium and voltage sensors with highly tunable photophysical and chemical properties, which can reliably detect single action potentials in cultured neurons.

PubMedSearch : Deo_2021_Nat.Chem.Biol_17_718
PubMedID: 33795886
Gene_locus related to this paper: rhoso-halo1

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Citations formats

Deo C, Abdelfattah AS, Bhargava HK, Berro AJ, Falco N, Farrants H, Moeyaert B, Chupanova M, Lavis LD, Schreiter ER (2021)
The HaloTag as a general scaffold for far-red tunable chemigenetic indicators
Nat Chemical Biology 17 :718

Deo C, Abdelfattah AS, Bhargava HK, Berro AJ, Falco N, Farrants H, Moeyaert B, Chupanova M, Lavis LD, Schreiter ER (2021)
Nat Chemical Biology 17 :718