| Title : Presence and release of SR-17 (chromogranin B(586-602)) in the porcine splenic nerve and its enzymatic degradation by CD26\/dipeptidyl peptidase IV - Depreitere_2002_Regul.Pept_106_71 |
| Author(s) : Depreitere J , Durinx C , Wang Z , Coen E , Lambeir AM , Scharpe S , De Potter W , Nouwen EJ |
| Ref : Regul Pept , 106 :71 , 2002 |
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Abstract :
Using the pig splenic nerve as a model, we investigated the proteolytic processing of porcine chromogranin B (CgB) during its axonal transport. An ELISA was developed for SR-17 (CgB(586-602)), a novel CgB-derived peptide, originally found in the adrenal medulla. The results demonstrate that CgB is processed in an early stage during its axonal transport. Immunohistochemical data, based on a rabbit anti-SR-17 antiserum, show that the spleen CgB/SR-17 is exclusively present in the nerve endings. No SR-17 immunoreactivity (IR) was found in splenocytes. We also provide evidence that SR-17 is co-released with noradrenaline (NA) upon electrical stimulation of the splenic nerve. Its release is frequency-dependent and strongly enhanced in the presence of the alpha-blocking agent phentolamine. In addition, we show that the new CgB-peptide can serve as a substrate for the lymphocyte surface glycoprotein CD26, also known as dipeptidyl peptidase IV (DPP IV), generating a new peptide ER-15 (CgB(588-602)). |
| PubMedSearch : Depreitere_2002_Regul.Pept_106_71 |
| PubMedID: 12047913 |
Depreitere J, Durinx C, Wang Z, Coen E, Lambeir AM, Scharpe S, De Potter W, Nouwen EJ (2002)
Presence and release of SR-17 (chromogranin B(586-602)) in the porcine splenic nerve and its enzymatic degradation by CD26\/dipeptidyl peptidase IV
Regul Pept
106 :71
Depreitere J, Durinx C, Wang Z, Coen E, Lambeir AM, Scharpe S, De Potter W, Nouwen EJ (2002)
Regul Pept
106 :71