| Title : Amelioration of cognitive deficit in zebrafish by an undescribed anthraquinone from Juglans regia L.: An in-silico, in-vitro and in-vivo approach - Devidas_2021_Eur.J.Pharmacol__174234 |
| Author(s) : Devidas SB , Rahmatkar SN , Singh R , Sendri N , Purohit R , Singh D , Bhandari P |
| Ref : European Journal of Pharmacology , :174234 , 2021 |
| Abstract : An undescribed anthraquinone assigned as 1-Hydroxy-5,5-dimethyl-5,6,7,8-tetrahydro-9,10-anthraquinone (compound 1) was isolated from ethylacetate extract of Juglans regia. The structure of the compound was established on the basis of 1D, 2D NMR (HSQC, HMBC, COSY), ESI-QTOF-MS/MS spectroscopy. The molecular docking studies of compound 1 indicated similar molecular interactions as that of co-crystalized inhibitor. Compound 1 showed hydrogen bonds with residues PHE295, GLY121, Pi- interactions with TYR 341, Pi-Pi interactions with HIS 447 residues, and Pi-alkyl with TRP86 and TYR 337. On the basis of in-silico interaction studies of compound 1 with proteins, it was tested using acetylcholinesterase inhibition assay, acrylamide-induced neurotoxicity test of zebrafish larva, and scopolamine-induced cognitive deficit model of adult zebrafish. The compound 1 showed potent acetylcholinesterase inhibition activity, prevented acrylamide-induced neurotoxicity and improved learning and memory functions in T-maze test. The results established compound 1 to be a potential neuroprotective natural product for amelioration of cognitive impairment. |
| ESTHER : Devidas_2021_Eur.J.Pharmacol__174234 |
| PubMedSearch : Devidas_2021_Eur.J.Pharmacol__174234 |
| PubMedID: 34090895 |
Devidas SB, Rahmatkar SN, Singh R, Sendri N, Purohit R, Singh D, Bhandari P (2021)
Amelioration of cognitive deficit in zebrafish by an undescribed anthraquinone from Juglans regia L.: An in-silico, in-vitro and in-vivo approach
European Journal of Pharmacology
:174234
Devidas SB, Rahmatkar SN, Singh R, Sendri N, Purohit R, Singh D, Bhandari P (2021)
European Journal of Pharmacology
:174234