Dilokpimol_2022_FEBS.Lett__

Feruloyl esterases (FAEs) and acetyl xylan esterases (AXEs) are important enzymes for plant biomass degradation and are both present in Carbohydrate Esterase family 1 (CE1) of the Carbohydrate-Active enZymes database. In this study, ten novel fungal CE1 enzymes from different subfamilies were heterologously produced and screened for their activity towards model and complex plant biomass substrates. CE1_1 enzymes possess AXE activity, while CE1_5 enzymes showed FAE activity. Two enzymes from CE1_2 and one from CE1_5 possess dual feruloyl/acetyl xylan esterase (FXE) activity, showing expansion of substrate specificity. The new FXEs from CE1 can efficiently release both feruloyl and acetyl residues from feruloylated xylan, making them particularly interesting novel components of industrial enzyme cocktails for plant biomass degradation. CE1_1 - AXE1B_MYCSE Axe1 MsAxe1 Corse1p7_016869 mycse-axe1b CE1_2 SF6 FAE1B_MYCSE Fxe1 CE1_5 SF5 FAE1C_MYCSE Fae1 MsFae1 Corse1p7_018453 mycse-fae1c CE1_5 SF5 FAE1E_MYCSE Fae2 MsFae2 Corse1p7_001265 mycse-fae1e Non-CE1 SF2 FAE1F_MYCSE Fae3 MsFae3 Corse1p7_018656 mycse-fae1f