Title : Modular organization of genes required for complex polyketide biosynthesis - Donadio_1991_Science_252_675 |
Author(s) : Donadio S , Staver MJ , McAlpine JB , Swanson SJ , Katz L |
Ref : Science , 252 :675 , 1991 |
Abstract :
In Saccharopolyspora erythraea, the genes that govern synthesis of the polyketide portion of the macrolide antibiotic erythromycin are organized in six repeated units that encode fatty acid synthase (FAS)-like activities. Each repeated unit is designated a module, and two modules are contained in a single open reading frame. A model for the synthesis of this complex polyketide is proposed, where each module encodes a functional synthase unit and each synthase unit participates specifically in one of the six FAS-like elongation steps required for formation of the polyketide. In addition, genetic organization and biochemical order of events appear to be colinear. Evidence for the model is provided by construction of a selected mutant and by isolation of a polyketide of predicted structure. |
PubMedSearch : Donadio_1991_Science_252_675 |
PubMedID: 2024119 |
Gene_locus related to this paper: sacer-ery3 |
Gene_locus | sacer-ery3 |
Donadio S, Staver MJ, McAlpine JB, Swanson SJ, Katz L (1991)
Modular organization of genes required for complex polyketide biosynthesis
Science
252 :675
Donadio S, Staver MJ, McAlpine JB, Swanson SJ, Katz L (1991)
Science
252 :675