Donadio_1991_Science_252_675

Reference

Title : Modular organization of genes required for complex polyketide biosynthesis - Donadio_1991_Science_252_675
Author(s) : Donadio S , Staver MJ , McAlpine JB , Swanson SJ , Katz L
Ref : Science , 252 :675 , 1991
Abstract :

In Saccharopolyspora erythraea, the genes that govern synthesis of the polyketide portion of the macrolide antibiotic erythromycin are organized in six repeated units that encode fatty acid synthase (FAS)-like activities. Each repeated unit is designated a module, and two modules are contained in a single open reading frame. A model for the synthesis of this complex polyketide is proposed, where each module encodes a functional synthase unit and each synthase unit participates specifically in one of the six FAS-like elongation steps required for formation of the polyketide. In addition, genetic organization and biochemical order of events appear to be colinear. Evidence for the model is provided by construction of a selected mutant and by isolation of a polyketide of predicted structure.

PubMedSearch : Donadio_1991_Science_252_675
PubMedID: 2024119
Gene_locus related to this paper: sacer-ery3

Related information

Gene_locus sacer-ery3

Citations formats

Donadio S, Staver MJ, McAlpine JB, Swanson SJ, Katz L (1991)
Modular organization of genes required for complex polyketide biosynthesis
Science 252 :675

Donadio S, Staver MJ, McAlpine JB, Swanson SJ, Katz L (1991)
Science 252 :675