Du_2012_Zhonghua.Yi.Xue.Za.Zhi_92_86

OBJECTIVE: To explore the effects of soluble epoxide hydrolase inhibitor (sEHi) on the expressions of fatty acid synthase (FASN) mRNA and protein in peripheral blood mononuclear cell (PBMCs) of patients with acute coronary syndrome (ACS) and to discuss the influences of sEHi in the regulated expression of FASN and inflammatory reaction.
METHODS: The hospitalized ACS patients were selected as the ACS group (n = 35) while the healthy normal subjects as the control group (n = 30). The levels of lipoproteins, fasting blood glucose, myocardial enzyme and hs-CRP (high-sensitive C-reactive protein) were measured within 24 hours after admission. Meanwhile the PBMCs were separated and cultured and then t-AUCB was added in various concentrations (0, 1, 10, 50, 100 micromol/L). The cellular expressions of FASN, IL-6 mRNA and protein were detected by real-time PCR (polymerase chain reaction) and Western blot respectively.
RESULTS: (1) The serum concentration of hs-CRP reached (5.6 +/- 4.1) mg/L in the ACS group. And it was obviously higher than (1.3 +/- 0.9) mg/L in the control group (P < 0.05). Compared with the control group, the expression levels of FASN, IL-6 mRNA and protein significantly increased in the ACS group (the relative expression amount of FASN mRNA: 1 vs 1.709 +/- 0.272, FASN protein: 0.407 +/- 0.065 vs 1.298 +/- 0.087; relative expression amount of IL-6 mRNA: 1 vs 2.302 +/- 0.200, IL-6 protein: 0.715 +/- 0.058 vs 1.146 +/- 0.083, P < 0.05). Moreover, the levels of FASN and IL-6 mRNA had positive correlations with the serum concentration of hs-CRP (r = 0.714, P < 0.01; r = 0.685, P < 0.01). (2) Compared with the control group (t-AUCB 0 micromol/L), 10, 50, 100 micromol/L t-AUCB had inhibited the expressions quantity of FASN, IL-6 mRNA and protein in PBMCs from the ACS group (P < 0.05). The relative expressions of FASN mRNA in t-AUCB 0, 10, 50, 100 micromol/L group were 1, 0.813 +/- 0.038, 0.564 +/- 0.100, 0.293 +/- 0.043 respectively. The relative expressions of FASN protein in t-AUCB 0, 10, 50 and 100 micromol/L group were 0.957 +/- 0.280, 0.935 +/- 0.275, 0.855 +/- 0.253, 0.685 +/- 0.206 respectively. CONCLUSION: The inflammatory level increases obviously in the ACS group. And the expression level of FASN in PBMCs is closely correlated with the inflammatory level in the ACS patients; t-AUCB may prevent the ruptures of atherosclerotic lesions by regulating FASN and inhibiting inflammatory reactions in ACS patients.