| Title : Identification and characterization of a triacylglycerol lipase in Arabidopsis homologous to mammalian acid lipases - El-Kouhen_2005_FEBS.Lett_579_6067 |
| Author(s) : El-Kouhen K , Blangy S , Ortiz E , Gardies AM , Ferte N , Arondel V |
| Ref : FEBS Letters , 579 :6067 , 2005 |
| Abstract : Triacylglycerol (TAG) lipases have been thoroughly characterized in mammals and microorganisms. By contrast, very little is known on plant TAG lipases. An Arabidopsis cDNA called AtLip1 (At2g15230), which exhibits strong homology to lysosomal acid lipase, was found to drive the synthesis of an active TAG lipase when expressed in the baculovirus system. The lipase had a maximal activity at pH 6 and the specific activity was estimated to be about 45 micromol min(-1) mg(-1) protein using triolein as a substrate. Knock-out mutant analysis showed no phenotype during germination indicating that this enzyme is fully dispensable for TAG storage breakdown during germination. Northern blot analyses indicated that the transcript is present in all tissues tested. |
| ESTHER : El-Kouhen_2005_FEBS.Lett_579_6067 |
| PubMedSearch : El-Kouhen_2005_FEBS.Lett_579_6067 |
| PubMedID: 16226259 |
| Gene_locus related to this paper: arath-At2g15230 |
| Gene_locus related to this paper: arath-At2g15230 |
El-Kouhen K, Blangy S, Ortiz E, Gardies AM, Ferte N, Arondel V (2005)
Identification and characterization of a triacylglycerol lipase in Arabidopsis homologous to mammalian acid lipases
FEBS Letters
579 :6067
El-Kouhen K, Blangy S, Ortiz E, Gardies AM, Ferte N, Arondel V (2005)
FEBS Letters
579 :6067