Farber_2001_Science_292_1385

Reference

Title : Genetic analysis of digestive physiology using fluorescent phospholipid reporters - Farber_2001_Science_292_1385
Author(s) : Farber SA , Pack M , Ho SY , Johnson ID , Wagner DS , Dosch R , Mullins MC , Hendrickson HS , Hendrickson EK , Halpern ME
Ref : Science , 292 :1385 , 2001
Abstract :

Zebrafish are a valuable model for mammalian lipid metabolism; larvae process lipids similarly through the intestine and hepatobiliary system and respond to drugs that block cholesterol synthesis in humans. After ingestion of fluorescently quenched phospholipids, endogenous lipase activity and rapid transport of cleavage products results in intense gall bladder fluorescence. Genetic screening identifies zebrafish mutants, such as fat free, that show normal digestive organ morphology but severely reduced phospholipid and cholesterol processing. Thus, fluorescent lipids provide a sensitive readout of lipid metabolism and are a powerful tool for identifying genes that mediate vertebrate digestive physiology.

PubMedSearch : Farber_2001_Science_292_1385
PubMedID: 11359013

Related information

Substrate PED-A1

Citations formats

Farber SA, Pack M, Ho SY, Johnson ID, Wagner DS, Dosch R, Mullins MC, Hendrickson HS, Hendrickson EK, Halpern ME (2001)
Genetic analysis of digestive physiology using fluorescent phospholipid reporters
Science 292 :1385

Farber SA, Pack M, Ho SY, Johnson ID, Wagner DS, Dosch R, Mullins MC, Hendrickson HS, Hendrickson EK, Halpern ME (2001)
Science 292 :1385