Fazio_2023_Methods.Mol.Biol_2576_299

The alpha,beta-hydrolase fold-containing protein 2 (ABHD2) is a serine hydrolase, responsible for the cleavage of endogenous 2-arachidonoylglycerol (2-AG). ABHD2 is activated by progesterone, thus, it is considered a nonnuclear receptor of this steroid hormone that terminates its biological effects. The products of ABHD2-catalyzed cleavage by the natural substrate 2-AG are glycerol and arachidonic acid; here, instead of 2-AG, the radioactive substrate 2-oleoyl-[(3)H]glycerol has been used as already done in various acylglycerol lipase activity assays. The amount of [(3)H]glycerol released allows to measure ABHD2 enzymatic activity.