Fernandez_1984_Neurochem.Res_9_1211

Reference

Title : Subcellular localization of acetylcholinesterase molecular forms in endplate regions of adult mammalian skeletal muscle - Fernandez_1984_Neurochem.Res_9_1211
Author(s) : Fernandez HL , Inestrosa NC , Stiles JR
Ref : Neurochemical Research , 9 :1211 , 1984
Abstract :

The characterization of individual acetylcholinesterase (AChE) molecular form subcellular pools in adult mammalian skeletal muscle is a critical point when considering such questions as the origin, assembly, and neurotrophic regulation of these molecules. By correlating the results of differential extraction, in vitro collagenase digestion, and in situ pharmacologic probes of AChE molecular forms in endplate regions of adult rat anterior gracilis muscle, we have shown that: 1) 4.0S (G1) and 6.0S (G2) AChE are predominantly membrane-bound and intracellular; if an extracellular and/or soluble fraction of these forms exists, it cannot be adequately resolved by our methods; 2) 9-11S (globular) AChE activity is distributed between internal and external pools, as well as membrane-associated and soluble fractions; 3) 16.0S (A12) AChE is not an integral membrane protein and exists both intracellularly (25-30%) and extracellularly (70-75%).

PubMedSearch : Fernandez_1984_Neurochem.Res_9_1211
PubMedID: 6504236

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Citations formats

Fernandez HL, Inestrosa NC, Stiles JR (1984)
Subcellular localization of acetylcholinesterase molecular forms in endplate regions of adult mammalian skeletal muscle
Neurochemical Research 9 :1211

Fernandez HL, Inestrosa NC, Stiles JR (1984)
Neurochemical Research 9 :1211