Ferrer_2013_Environ.Microbiol_15_211

Reference

Title : Microbiota from the distal guts of lean and obese adolescents exhibit partial functional redundancy besides clear differences in community structure - Ferrer_2013_Environ.Microbiol_15_211
Author(s) : Ferrer M , Ruiz A , Lanza F , Haange SB , Oberbach A , Till H , Bargiela R , Campoy C , Segura MT , Richter M , von Bergen M , Seifert J , Suarez A
Ref : Environ Microbiol , 15 :211 , 2013
Abstract :

Recent research has disclosed a tight connection between obesity, metabolic gut microbial activities and host health. Obtaining a complete understanding of this relationship remains a major goal. Here, we conducted a comparative metagenomic and metaproteomic investigation of gut microbial communities in faecal samples taken from an obese and a lean adolescent. By analysing the diversity of 16S rDNA amplicons (10% operational phylogenetic units being common), 22 Mbp of consensus metagenome sequences (~70% common) and the expression profiles of 613 distinct proteins (82% common), we found that in the obese gut, the total microbiota was more abundant on the phylum Firmicutes (94.6%) as compared with Bacteroidetes (3.2%), although the metabolically active microbiota clearly behaves in a more homogeneous manner with both contributing equally. The lean gut showed a remarkable shift towards Bacteroidetes (18.9% total 16S rDNA), which become the most active fraction (81% proteins). Although the two gut communities maintained largely similar gene repertoires and functional profiles, improved pili- and flagella-mediated host colonization and improved capacity for both complementary aerobic and anaerobic de novo B(12) synthesis, 1,2-propanediol catabolism (most likely participating in de novo B(12) synthesis) and butyrate production were observed in the obese gut, whereas bacteria from lean gut seem to be more engaged in vitamin B(6) synthesis. Furthermore, this study provides functional evidence that variable combinations of species from different phyla could 'presumptively' fulfil overlapping and/or complementary functional roles required by the host, a scenario where minor bacterial taxa seem to be significant active contributors.

PubMedSearch : Ferrer_2013_Environ.Microbiol_15_211
PubMedID: 22891823
Gene_locus related to this paper: 9bact-d1ped1 , 9bact-d1pg85 , 9firm-a5z5y4 , 9firm-a5z5y5 , bacun-a7v041 , 9bact-d1p9d7

Related information

Gene_locus 9bact-d1ped1    9bact-d1pg85    9firm-a5z5y4    9firm-a5z5y5    bacun-a7v041    9bact-d1p9d7
Gene_locus_frgt 9bact-d1ped1    9bact-d1pg85    9firm-a5z5y4    9firm-a5z5y5    bacun-a7v041    9bact-d1p9d7    9zzzz-k1upf2    9zzzz-k1sjs7    9zzzz-k1t8k3    9zzzz-k1t943    9zzzz-k1u6d8    9zzzz-k1v7u9

Citations formats

Ferrer M, Ruiz A, Lanza F, Haange SB, Oberbach A, Till H, Bargiela R, Campoy C, Segura MT, Richter M, von Bergen M, Seifert J, Suarez A (2013)
Microbiota from the distal guts of lean and obese adolescents exhibit partial functional redundancy besides clear differences in community structure
Environ Microbiol 15 :211

Ferrer M, Ruiz A, Lanza F, Haange SB, Oberbach A, Till H, Bargiela R, Campoy C, Segura MT, Richter M, von Bergen M, Seifert J, Suarez A (2013)
Environ Microbiol 15 :211