| Title : DspA from Strongylocentrotus purpuratus: The first biochemically characterized haloalkane dehalogenase of non-microbial origin - Fortova_2013_Biochimie_95_2091 |
| Author(s) : Fortova A , Sebestova E , Stepankova V , Koudelakova T , Palkova L , Damborsky J , Chaloupkova R |
| Ref : Biochimie , 95 :2091 , 2013 |
|
Abstract :
Haloalkane dehalogenases are known as bacterial enzymes cleaving a carbon-halogen bond in halogenated compounds. Here we report the first biochemically characterized non-microbial haloalkane dehalogenase DspA from Strongylocentrotus purpuratus. The enzyme shows a preference for terminally brominated hydrocarbons and enantioselectivity towards beta-brominated alkanes. Moreover, we identified other putative haloalkane dehalogenases of eukaryotic origin, representing targets for future experiments to discover dehalogenases with novel catalytic properties. |
| PubMedSearch : Fortova_2013_Biochimie_95_2091 |
| PubMedID: 23939220 |
| Gene_locus related to this paper: strpu-h3hrw2 |
| Gene_locus | strpu-h3hrw2 |
Fortova A, Sebestova E, Stepankova V, Koudelakova T, Palkova L, Damborsky J, Chaloupkova R (2013)
DspA from Strongylocentrotus purpuratus: The first biochemically characterized haloalkane dehalogenase of non-microbial origin
Biochimie
95 :2091
Fortova A, Sebestova E, Stepankova V, Koudelakova T, Palkova L, Damborsky J, Chaloupkova R (2013)
Biochimie
95 :2091