Frei_2022_Nat.Methods_19_65

Reference

Title : Engineered HaloTag variants for fluorescence lifetime multiplexing - Frei_2022_Nat.Methods_19_65
Author(s) : Frei MS , Tarnawski M , Roberti MJ , Koch B , Hiblot J , Johnsson K
Ref : Nat Methods , 19 :65 , 2022
Abstract :

Self-labeling protein tags such as HaloTag are powerful tools that can label fusion proteins with synthetic fluorophores for use in fluorescence microscopy. Here we introduce HaloTag variants with either increased or decreased brightness and fluorescence lifetime compared with HaloTag7 when labeled with rhodamines. Combining these HaloTag variants enabled live-cell fluorescence lifetime multiplexing of three cellular targets in one spectral channel using a single fluorophore and the generation of a fluorescence lifetime-based biosensor. Additionally, the brightest HaloTag variant showed up to 40% higher brightness in live-cell imaging applications.

PubMedSearch : Frei_2022_Nat.Methods_19_65
PubMedID: 34916672
Gene_locus related to this paper: rhoso-halo1

Citations formats

Frei MS, Tarnawski M, Roberti MJ, Koch B, Hiblot J, Johnsson K (2022)
Engineered HaloTag variants for fluorescence lifetime multiplexing
Nat Methods 19 :65

Frei MS, Tarnawski M, Roberti MJ, Koch B, Hiblot J, Johnsson K (2022)
Nat Methods 19 :65