| Title : Development of ESI-MS-based continuous enzymatic assay for real-time monitoring of enzymatic reactions of acetylcholinesterase - Fu_2015_J.Chromatogr.B.Analyt.Technol.Biomed.Life.Sci_990_169 |
| Author(s) : Fu Q , Tang J , Cui M , Zheng Z , Liu Z , Liu S |
| Ref : Journal of Chromatography B Analyt Technol Biomed Life Sciences , 990 :169 , 2015 |
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Abstract :
The continuous enzymatic assay based on ESI-MS was developed to real-time monitoring of enzymatic reactions of acetylcholinesterase (AChE). The changes of product concentrations were continuously measured. Calibration curves were established for quantitative calculation. By this method, the Michaelis constant (Km) of acetylcholinesterase was determined to be 70.60+/-0.93muM and Huperzine A as an effective inhibitor of acetylcholinesterase displayed a mixed inhibition with competitive and noncompetitive inhibition behaviors. The half maximal inhibitory concentration (IC50) and inhibition constant (Ki) value of Huperzine A were also calculated as 48.51+/-1.16nM and 26.73+/-0.27nM, respectively. This method provides the rapid and accurate ways to monitor enzyme reactions. |
| PubMedSearch : Fu_2015_J.Chromatogr.B.Analyt.Technol.Biomed.Life.Sci_990_169 |
| PubMedID: 25875590 |
Fu Q, Tang J, Cui M, Zheng Z, Liu Z, Liu S (2015)
Development of ESI-MS-based continuous enzymatic assay for real-time monitoring of enzymatic reactions of acetylcholinesterase
Journal of Chromatography B Analyt Technol Biomed Life Sciences
990 :169
Fu Q, Tang J, Cui M, Zheng Z, Liu Z, Liu S (2015)
Journal of Chromatography B Analyt Technol Biomed Life Sciences
990 :169