Goettig_2005_J.Biol.Chem_280_33387

Reference

Title : X-ray snapshots of peptide processing in mutants of tricorn-interacting factor F1 from Thermoplasma acidophilum - Goettig_2005_J.Biol.Chem_280_33387
Author(s) : Goettig P , Brandstetter H , Groll M , Gohring W , Konarev PV , Svergun DI , Huber R , Kim JS
Ref : Journal of Biological Chemistry , 280 :33387 , 2005
Abstract :

The tricorn-interacting factor F1 of the archaeon Thermoplasma acidophilum cleaves small hydrophobic peptide products of the proteasome and tricorn protease. F1 mutants of the active site residues that are involved in substrate recognition and catalysis displayed distinct activity patterns toward fluorogenic test substrates. Crystal structures of the mutant proteins complexed with peptides Phe-Leu, Pro-Pro, or Pro-Leu-Gly-Gly showed interaction of glutamates 213 and 245 with the N termini of the peptides and defined the S1 and S1' sites and the role of the catalytic residues. Evidence was found for processive peptide cleavage in the N-to-C direction, whereby the P1' product is translocated into the S1 site. A functional interaction of F1 with the tricorn protease was observed with the inactive F1 mutant G37A. Moreover, small angle x-ray scattering measurements for tricorn and inhibited F1 have been interpreted as formation of transient and substrate-induced complexes.

PubMedSearch : Goettig_2005_J.Biol.Chem_280_33387
PubMedID: 15994304
Gene_locus related to this paper: theac-pip

Related information

Inhibitor PHK
Substrate PHK    Phe-Ala    Pro-Pro    Pro-Leu-Gly-Gly    Ala-Phe    Phe-Leu
Gene_locus PHK    Phe-Ala    Pro-Pro    Pro-Leu-Gly-Gly    Ala-Phe    Phe-Leu    theac-pip
Family PHK    Phe-Ala    Pro-Pro    Pro-Leu-Gly-Gly    Ala-Phe    Phe-Leu    theac-pip    Proline_iminopeptidase
Structure PHK    Phe-Ala    Pro-Pro    Pro-Leu-Gly-Gly    Ala-Phe    Phe-Leu    theac-pip    Proline_iminopeptidase    1XQV    1XQX    1XRL    1XRN    1XRP    1XRR    1XQW    1XQY    1XRM    1XRO    1XRQ

Citations formats

Goettig P, Brandstetter H, Groll M, Gohring W, Konarev PV, Svergun DI, Huber R, Kim JS (2005)
X-ray snapshots of peptide processing in mutants of tricorn-interacting factor F1 from Thermoplasma acidophilum
Journal of Biological Chemistry 280 :33387

Goettig P, Brandstetter H, Groll M, Gohring W, Konarev PV, Svergun DI, Huber R, Kim JS (2005)
Journal of Biological Chemistry 280 :33387