Gorne-Tschelnokow_1993_Eur.J.Biochem_213_1235

The secondary structure of the acetylcholinesterase and its temperature behaviour have been investigated using Fourier-transform infrared (FTIR) spectroscopy. The data are compared to the structure obtained by X-ray analysis of the crystalline enzyme. The secondary structure was determined using the spectral features observed in the amide-I band (H2O buffer) and amide-I' band (D2O buffer) at 1600-1700 cm-1, taking advantage of resolution-enhancement techniques along with least-squares band-fitting procedures. The relative amounts of different secondary-structure elements, 34-36% for alpha-helices, 19-25% for beta-sheets, 15-16% for turns and 13-17% for irregular structures, were estimated. These data, obtained with the enzyme in solution, correlate well with X-ray data of the crystalline protein [Sussman, J. L., Hard, M., Frolow, F., Oefner, C., Goldman, A., Toker, L. & Silman, I. (1991) Science 253, 872-879]. These results are also in good agreement with those obtained by computing the psi and phi angles of the peptide backbone using the Kabsch and Sanders method [Kabsch, W. & Sanders, C. (1983) Biopolymers 22, 2577-2637]. In conjunction with the X-ray data, two bands in the FTIR spectra were assigned to different populations of long and short alpha-helices. Until now this phenomenon has only been described by theoretical calculations [Nevskaya, N. A. & Chirgadze, Yu. N. (1976) Biopolymers 15, 637-648]. The relationship between the thermally induced loss of enzyme activity and secondary-structure changes has also been investigated. The decrease in enzyme activity to zero at 30-40 degrees C was accompanied only by minor changes in the secondary structure. At 55-60 degrees C, denaturation of AChE occurs. In this temperature range, all bands assigned to the various secondary-structure elements abruptly disappear in a co-operative and irreversible manner, whereas the beta-aggregation bands (at 1622 cm-1 and the corresponding high-frequency band) increase in intensity at the same rate.