Grognet_1993_Arch.Toxicol_67_66

Reference

Title : Production and characterization of antibodies directed against organophosphorus nerve agent VX - Grognet_1993_Arch.Toxicol_67_66
Author(s) : Grognet JM , Ardouin T , Istin M , Vandais A , Noel JP , Rima G , Satge J , Pradel C , Sentenac-Roumanou H , Lion C
Ref : Archives of Toxicology , 67 :66 , 1993
Abstract :

A strategy is described to raise high-affinity antibodies directed against the organophosphorus nerve agent VX [O-ethyl S-(2-diisopropylamino)ethyl)methyl phosponothionate]. Ten chemical derivatives of VX (haptens) have been synthesized. Their structures differ principally from VX structure by substitution of S-atom by an O-atom or CH2-group and by introduction of a reactive group (carboxylic acid, arylamine or primary amine) on the O-ethyl side chain. None of these haptens, except one, exhibit potential toxicity as tested by their inhability to inhibit acetylcholinesterase (E.C. 3.1.1.7.). After coupling with a protein carrier, they were injected intradermally to rabbits. Nine of these immunogenic conjugates led to the appearance of antibodies able to bind VX in a competitive solid phase immunoassay. The apparent titer and affinity of the antisera differed greatly depending on the hapten used. The highest affinity (9 nM) was observed with the VX derivative bearing O-S substitution and O-ethyl-carboxylic side chains. The antibodies appear specific for VX, since cross-reactivity with other nerve agents (Soman, Sarin or Tabun) was low. However, two haptens elicited antibodies with affinity to Soman or Sarin in the micromolar range. Antibodies were able to neutralize VX inhibition of acetylcholinesterase in vitro but not in vivo.

PubMedSearch : Grognet_1993_Arch.Toxicol_67_66
PubMedID: 8452483

Related information

Inhibitor VX

Citations formats

Grognet JM, Ardouin T, Istin M, Vandais A, Noel JP, Rima G, Satge J, Pradel C, Sentenac-Roumanou H, Lion C (1993)
Production and characterization of antibodies directed against organophosphorus nerve agent VX
Archives of Toxicology 67 :66

Grognet JM, Ardouin T, Istin M, Vandais A, Noel JP, Rima G, Satge J, Pradel C, Sentenac-Roumanou H, Lion C (1993)
Archives of Toxicology 67 :66